Detailed information NTG0290064

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EKH72_RS15020
• Genome accession: NZ_CP034565
• Coordinates: 2920877..2923081 (+)
• Length: 734 a.a.
• NCBI ID: WP_078231457.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain D3112
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2915877..2928081

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EKH72_RS14975	rplV	2916546..2916878 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
EKH72_RS14980	rpsS	2916889..2917167 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
EKH72_RS14985	rplB	2917189..2918013 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
EKH72_RS14990	rplW	2918029..2918331 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
EKH72_RS14995	rplD	2918328..2918930 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
EKH72_RS15000	rplC	2918948..2919577 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
EKH72_RS15005	rpsJ	2919592..2919903 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
EKH72_RS15010	rpmE	2920361..2920582 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
EKH72_RS15020	priA	2920877..2923081 (+)	2205	WP_078231457.1	primosomal protein N'	Machinery gene
EKH72_RS15025	cytR	2923414..2924421 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
EKH72_RS15030	ftsN	2924598..2925143 (+)	546	WP_005481405.1	cell division protein FtsN	-
EKH72_RS15035	hslV	2925308..2925859 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
EKH72_RS15040	hslU	2925883..2927214 (+)	1332	WP_005457198.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82261.79 Da
• Isoelectric Point: 8.5714

>NTDB_id=290064 EKH72_RS15020 WP_078231457.1 2920877..2923081(+) (priA) [Vibrio parahaemolyticus strain D3112]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWSEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=290064 EKH72_RS15020 WP_078231457.1 2920877..2923081(+) (priA) [Vibrio parahaemolyticus strain D3112]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCCAAAGATCAACTCAAGCCAATCAAAGCGGTTCTTGATTCTCAGCCGGTATGGTCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAATTCTACCAGTACCCACTTGGAGATACGCTACACAATGCGATGCCGGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACAGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTGGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAGGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCTTTGGTTTTAGTACCAGAGATTGGCTTAACACCGCAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACTCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTGTTTCCCGAATACAA
AGCTATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCTTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCAAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.514	100	0.439
priA	Bacillus subtilis subsp. subtilis str. 168	34.131	100	0.369