Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DDT10_RS08780 Genome accession   NZ_CP029071
Coordinates   1804014..1804448 (+) Length   144 a.a.
NCBI ID   WP_053573457.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain ALB79     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1799014..1809448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDT10_RS08775 (DDT10_08775) - 1802514..1803725 (-) 1212 WP_039254928.1 cytochrome P450 -
  DDT10_RS08780 (DDT10_08780) nucA/comI 1804014..1804448 (+) 435 WP_053573457.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DDT10_RS08785 (DDT10_08785) - 1804508..1805263 (+) 756 WP_003154084.1 YoaK family protein -
  DDT10_RS08790 (DDT10_08790) - 1805292..1805654 (-) 363 WP_053573458.1 hypothetical protein -
  DDT10_RS08795 (DDT10_08795) - 1805847..1807175 (-) 1329 WP_015417518.1 S8 family peptidase -
  DDT10_RS08800 (DDT10_08800) - 1807354..1807587 (+) 234 WP_015239897.1 hypothetical protein -
  DDT10_RS08805 (DDT10_08805) - 1807843..1808550 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  DDT10_RS08810 (DDT10_08810) - 1808610..1809062 (+) 453 WP_014305039.1 OsmC family protein -
  DDT10_RS08815 (DDT10_08815) - 1809076..1809429 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15548.46 Da        Isoelectric Point: 7.2418

>NTDB_id=290035 DDT10_RS08780 WP_053573457.1 1804014..1804448(+) (nucA/comI) [Bacillus amyloliquefaciens strain ALB79]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=290035 DDT10_RS08780 WP_053573457.1 1804014..1804448(+) (nucA/comI) [Bacillus amyloliquefaciens strain ALB79]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.829

81.25

0.486

Multiple sequence alignment