Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DDG65_RS08425 Genome accession   NZ_CP029032
Coordinates   1655071..1656141 (-) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   -
Organism   Staphylococcus aureus strain OXLIM     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1630942..1657087 1655071..1656141 within 0


Gene organization within MGE regions


Location: 1630942..1657087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDG65_RS08275 - 1630942..1631478 (-) 537 WP_077442703.1 IS30 family transposase -
  DDG65_RS08280 - 1631459..1631962 (-) 504 WP_000746375.1 transposase -
  DDG65_RS08285 - 1632071..1632427 (-) 357 WP_001255379.1 cystatin-like fold lipoprotein -
  DDG65_RS08290 - 1632483..1633073 (-) 591 WP_000810443.1 hypothetical protein -
  DDG65_RS08295 - 1633080..1634126 (-) 1047 WP_000247465.1 CHAP domain-containing protein -
  DDG65_RS08300 - 1634116..1635963 (-) 1848 WP_000681154.1 CD3337/EF1877 family mobilome membrane protein -
  DDG65_RS08305 - 1635968..1637326 (-) 1359 WP_001251211.1 FtsK/SpoIIIE domain-containing protein -
  DDG65_RS08310 - 1637380..1639875 (-) 2496 WP_001049264.1 ATP-binding protein -
  DDG65_RS08315 - 1639910..1640293 (-) 384 WP_000358144.1 TcpE family conjugal transfer membrane protein -
  DDG65_RS08320 - 1640305..1640565 (-) 261 WP_000015639.1 TcpD family membrane protein -
  DDG65_RS08325 - 1640570..1641625 (-) 1056 WP_000692003.1 conjugal transfer protein -
  DDG65_RS08330 - 1641686..1642777 (-) 1092 WP_000172939.1 replication initiation factor domain-containing protein -
  DDG65_RS08335 - 1642952..1643254 (-) 303 WP_000386891.1 hypothetical protein -
  DDG65_RS08340 - 1643268..1643588 (-) 321 WP_000805733.1 DUF961 family protein -
  DDG65_RS08345 - 1643739..1644023 (-) 285 WP_000134545.1 hypothetical protein -
  DDG65_RS08350 efp 1644418..1644975 (-) 558 WP_000626504.1 elongation factor P -
  DDG65_RS08355 - 1645001..1646062 (-) 1062 WP_000087107.1 M24 family metallopeptidase -
  DDG65_RS08360 - 1646167..1646748 (+) 582 WP_000737686.1 hypothetical protein -
  DDG65_RS08365 - 1646762..1646980 (+) 219 WP_001244298.1 SA1362 family protein -
  DDG65_RS08370 - 1647044..1647874 (-) 831 WP_000141108.1 biotin/lipoate A/B protein ligase family protein -
  DDG65_RS08375 - 1648032..1648418 (+) 387 WP_001276571.1 rhodanese-like domain-containing protein -
  DDG65_RS08380 gcvPB 1648783..1650255 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DDG65_RS08385 gcvPA 1650248..1651594 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DDG65_RS08390 gcvT 1651614..1652705 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DDG65_RS08395 - 1652864..1653388 (-) 525 WP_001015117.1 shikimate kinase -
  DDG65_RS08400 - 1653378..1653524 (-) 147 WP_001789879.1 hypothetical protein -
  DDG65_RS08405 comGF 1653621..1654118 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DDG65_RS08410 comGE 1654036..1654335 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DDG65_RS08415 comGD 1654322..1654768 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  DDG65_RS08420 comGC 1654746..1655057 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DDG65_RS08425 comGB 1655071..1656141 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  DDG65_RS08430 comGA 1656113..1657087 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=289451 DDG65_RS08425 WP_000775708.1 1655071..1656141(-) (comGB) [Staphylococcus aureus strain OXLIM]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=289451 DDG65_RS08425 WP_000775708.1 1655071..1656141(-) (comGB) [Staphylococcus aureus strain OXLIM]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997

Multiple sequence alignment