Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EJH97_RS00430 Genome accession   NZ_CP034389
Coordinates   101576..102244 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain RS571     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 96576..107244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJH97_RS00405 (EJH97_00405) pyrE 97101..97742 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EJH97_RS00410 (EJH97_00410) - 98040..98999 (+) 960 WP_022646277.1 reverse transcriptase family protein -
  EJH97_RS00415 (EJH97_00415) slmA 99045..99641 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  EJH97_RS00420 (EJH97_00420) dut 99748..100206 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EJH97_RS00425 (EJH97_00425) coaBC 100184..101404 (-) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EJH97_RS00430 (EJH97_00430) radC 101576..102244 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EJH97_RS00435 (EJH97_00435) rpmB 102461..102697 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EJH97_RS00440 (EJH97_00440) rpmG 102718..102885 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EJH97_RS00445 (EJH97_00445) mutM 102983..103792 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EJH97_RS00450 (EJH97_00450) coaD 103831..104310 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EJH97_RS00455 (EJH97_00455) waaA 104318..105595 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EJH97_RS00460 (EJH97_00460) rfaQ 106008..107066 (+) 1059 WP_000360395.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=289322 EJH97_RS00430 WP_001297375.1 101576..102244(+) (radC) [Escherichia coli strain RS571]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=289322 EJH97_RS00430 WP_001297375.1 101576..102244(+) (radC) [Escherichia coli strain RS571]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTTACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTAATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496