Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DCE79_RS18025 Genome accession   NZ_CP029002
Coordinates   3664912..3666285 (-) Length   457 a.a.
NCBI ID   WP_108714319.1    Uniprot ID   A0A2S1H6A8
Organism   Lysinibacillus sp. 2017     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3659912..3671285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCE79_RS18000 (DCE79_18000) gltX 3660196..3661656 (-) 1461 WP_108714315.1 glutamate--tRNA ligase -
  DCE79_RS18005 (DCE79_18005) ispF 3661778..3662254 (-) 477 WP_108714316.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DCE79_RS18010 (DCE79_18010) ispD 3662387..3663076 (-) 690 WP_108714317.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DCE79_RS18015 (DCE79_18015) - 3663090..3664166 (-) 1077 WP_108714318.1 PIN/TRAM domain-containing protein -
  DCE79_RS18020 (DCE79_18020) - 3664487..3664809 (+) 323 Protein_3453 hypothetical protein -
  DCE79_RS18025 (DCE79_18025) radA 3664912..3666285 (-) 1374 WP_108714319.1 DNA repair protein RadA Machinery gene
  DCE79_RS18030 (DCE79_18030) - 3666419..3668863 (-) 2445 WP_108714320.1 ATP-dependent Clp protease ATP-binding subunit -
  DCE79_RS18035 (DCE79_18035) - 3668863..3669957 (-) 1095 WP_108714321.1 protein arginine kinase -
  DCE79_RS18040 (DCE79_18040) - 3669954..3670496 (-) 543 WP_108714322.1 UvrB/UvrC motif-containing protein -
  DCE79_RS18045 (DCE79_18045) - 3670510..3670974 (-) 465 WP_108714323.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50258.90 Da        Isoelectric Point: 7.4029

>NTDB_id=289194 DCE79_RS18025 WP_108714319.1 3664912..3666285(-) (radA) [Lysinibacillus sp. 2017]
MAKKKSKFMCSSCGYEAAKWMGRCPGCGEWNTMNEEIEIISKGVRGAFQHSTTTTKATPIINVEVQEETRIVTQMGEFNR
VLGGGIVPGSLVLIGGDPGIGKSTLLLQISALLSNQGKRVLYISGEESVRQTKLRAERLGVKSPELYIYSETNLEFLNQT
IEEVQPKFVIVDSIQTVHHPEVTSAPGSVSQVRECTAELMRIAKTKNIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFEG
ERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAPGSTVVASMEGTRPILVEIQSLVTPTSFNY
PKRMATGVDQNRVQLLMAVLEKRMGMMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRPTDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRAIIPASNIGGWDFPEGIQIVGVETISDALRSSFQDL

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=289194 DCE79_RS18025 WP_108714319.1 3664912..3666285(-) (radA) [Lysinibacillus sp. 2017]
ATGGCGAAGAAAAAATCGAAGTTTATGTGTAGTAGCTGTGGCTATGAAGCGGCAAAATGGATGGGGCGCTGTCCAGGCTG
TGGCGAATGGAATACGATGAATGAAGAAATAGAAATCATTTCAAAGGGAGTTCGCGGAGCATTCCAACATTCTACAACGA
CAACAAAGGCGACACCAATTATTAATGTGGAAGTGCAAGAGGAAACACGTATTGTTACGCAAATGGGTGAATTTAACCGT
GTACTTGGTGGTGGGATTGTACCAGGTTCACTTGTGTTAATTGGTGGGGATCCTGGGATTGGGAAATCGACATTACTTTT
ACAGATTTCTGCGCTTTTATCAAACCAAGGAAAACGAGTCCTCTATATTTCAGGGGAGGAATCAGTACGCCAAACAAAGT
TACGCGCGGAACGTTTAGGCGTGAAATCTCCGGAGCTTTACATATACTCTGAAACGAATTTAGAGTTTTTAAATCAAACA
ATTGAAGAAGTTCAACCGAAATTTGTGATTGTCGATTCCATTCAAACTGTTCATCACCCAGAAGTCACAAGTGCACCAGG
AAGTGTGTCACAAGTGCGTGAATGTACCGCAGAATTAATGCGCATTGCGAAAACGAAAAACATTGCGATTTTCTTAGTTG
GTCACGTAACAAAAGAAGGGCAAATTGCAGGTCCGCGTATTTTAGAACATATGGTGGATACCGTGCTTTATTTTGAAGGC
GAGCGTCATCATAATCACCGTATTTTAAGAAGTCAAAAAAACCGTTTTGGTTCAACAAATGAAATTGCGATTTTTGAAAT
GCTACAAGGTGGATTGAAAGAAGTATTAAATCCGTCTGAGCTATTTTTACAGGAGCGTTCACAAGGGGCACCTGGCTCAA
CGGTTGTAGCATCCATGGAAGGAACACGTCCAATTCTAGTAGAAATCCAATCTCTTGTGACACCAACCAGTTTTAATTAT
CCAAAGCGTATGGCAACAGGAGTCGATCAAAACCGTGTGCAATTGTTAATGGCTGTGCTTGAAAAACGTATGGGAATGAT
GCTACAAGCGCAAGATGCTTATATTAAAGTTGCAGGCGGTGTGAAATTAGATGAACCTGCCATTGATTTAGCGGTGTTAA
CGAGCATTGTTTCAAGCTTTAAAGACCAGGCTGTTCGTCCGACGGATTGTTTTATTGGTGAGGTTGGTTTAACGGGAGAG
GTACGCCGTGTATCGCGTATTGAACAACGTGTACAAGAAGCAGCAAAATTAGGGTTTAAGCGTGCGATTATTCCTGCGTC
AAATATCGGAGGATGGGATTTCCCTGAAGGAATTCAAATCGTTGGTGTCGAAACAATTAGTGATGCTTTACGATCTTCAT
TTCAAGATTTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-428)

AAA domain (AAA_25)

(79-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S1H6A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.614

99.781

0.705
  radA Streptococcus pneumoniae Rx1

61.926

100

0.619
  radA Streptococcus pneumoniae D39

61.926

100

0.619
  radA Streptococcus pneumoniae R6

61.926

100

0.619
  radA Streptococcus pneumoniae TIGR4

61.926

100

0.619
  radA Streptococcus mitis NCTC 12261

61.926

100

0.619
  radA Streptococcus mitis SK321

61.926

100

0.619

Multiple sequence alignment