Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DCN14_RS25245 Genome accession   NZ_CP028963
Coordinates   2285219..2286595 (-) Length   458 a.a.
NCBI ID   WP_096507315.1    Uniprot ID   A0A2A4C332
Organism   Burkholderia sp. IDO3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2280219..2291595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCN14_RS25225 (DCN14_25225) cls 2280526..2281965 (+) 1440 WP_096507319.1 cardiolipin synthase -
  DCN14_RS25230 (DCN14_25230) - 2282097..2282576 (-) 480 WP_096507318.1 glutathione peroxidase -
  DCN14_RS25235 (DCN14_25235) - 2282713..2284644 (+) 1932 WP_096507317.1 ATP-binding cassette domain-containing protein -
  DCN14_RS25240 (DCN14_25240) - 2284803..2285069 (-) 267 WP_096507316.1 DUF2866 domain-containing protein -
  DCN14_RS25245 (DCN14_25245) radA 2285219..2286595 (-) 1377 WP_096507315.1 DNA repair protein RadA Machinery gene
  DCN14_RS25250 (DCN14_25250) alr 2286607..2287677 (-) 1071 WP_096507314.1 alanine racemase -
  DCN14_RS25255 (DCN14_25255) lplT 2287896..2289203 (+) 1308 WP_096507313.1 lysophospholipid transporter LplT -
  DCN14_RS25260 (DCN14_25260) thiD 2289262..2290071 (+) 810 WP_096507312.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  DCN14_RS25265 (DCN14_25265) - 2290090..2291049 (-) 960 WP_096507311.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48766.28 Da        Isoelectric Point: 6.9501

>NTDB_id=289039 DCN14_RS25245 WP_096507315.1 2285219..2286595(-) (radA) [Burkholderia sp. IDO3]
MAKQKTVYVCSECGGQTPKWQGQCPSCQAWNTLVESVAESPAAHRFQSLAKRAPVQRLVDIEAADVPRFTTGIGEFDRVL
GGGLVPGGVVLIGGDPGIGKSTLLLQSLADIASERRALYISGEESAAQIALRAQRLALLDGGAPAAELKLLAEIQLEKIQ
AAIDAERPDVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEVQALVDTAHV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTIALIPKANAPKQAIEGLKVMAVERLEQAIDSVRDLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=289039 DCN14_RS25245 WP_096507315.1 2285219..2286595(-) (radA) [Burkholderia sp. IDO3]
GTGGCCAAACAGAAAACCGTCTACGTCTGCAGCGAGTGCGGCGGACAAACACCGAAGTGGCAGGGGCAATGCCCGTCGTG
CCAGGCCTGGAACACGCTCGTCGAATCGGTTGCCGAGTCGCCGGCGGCCCATCGCTTCCAGTCGCTCGCGAAGCGCGCGC
CCGTGCAGCGTCTGGTCGACATCGAGGCGGCCGACGTGCCGCGTTTCACGACGGGGATCGGCGAATTCGACCGCGTGCTC
GGCGGCGGGCTGGTGCCGGGCGGCGTCGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGTC
GCTCGCGGACATCGCGAGCGAGCGGCGCGCACTCTATATCAGTGGCGAGGAATCGGCCGCGCAGATCGCGTTGCGCGCGC
AACGGCTCGCGCTGCTCGACGGCGGCGCGCCGGCGGCCGAGCTGAAGCTGCTCGCCGAGATCCAGCTCGAGAAGATCCAG
GCGGCGATCGATGCCGAACGGCCGGACGTCGCCGTCATCGACTCGATCCAGACCGTCTATTCGGAGGCGCTGACGTCGGC
GCCCGGTTCGGTCGCGCAGGTGCGCGAATGCGCGGCGCAGCTCACGCGCATCGCGAAGCAGTCCGGCACCGCGATCATCA
TGGTCGGGCACGTGACGAAGGAGGGCAACCTTGCCGGTCCGCGCGTGCTCGAGCACATCGTCGACACGGTGCTGTACTTC
GAGGGCGACACCCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAACTCGGCGTGTT
CGCGATGACCGAGCGTGGGCTGCGCGGCGTCGCGAATCCTTCCGCGCTTTTCCTGTCGCAGCATGAGCAAGTCGTGCCGG
GCTCGTGCGTGCTCGTCACGCAGGAAGGCACGCGGCCGCTGCTGGTCGAGGTCCAGGCGCTCGTCGACACGGCCCACGTG
CCGAATCCGCGCCGGCTCGCGGTCGGCCTCGAGCAGAACCGGCTCGCGATGCTGCTCGCGGTGCTGCACCGTCATGCGGG
CATCGCGTGCTTCGACCAGGACGTGTTTCTCAACGCGGTCGGCGGCGTGAAGATCACGGAGCCGGCCGCCGACCTCGCGG
TGCTGCTCGCGATCCACTCGTCGATGCGCAACAAGGCGCTGCCGAAGGGGTTGATCGTGTTCGGCGAAGTCGGGCTGGCC
GGTGAAATCCGGCCATCCCCGCGCGGGCAGGAGCGCTTGCGCGAGGCCGCGAAGCTCGGCTTCACGATTGCGCTGATTCC
GAAGGCGAATGCGCCGAAGCAGGCGATCGAAGGGCTGAAGGTGATGGCCGTCGAGCGGCTCGAACAGGCGATCGACAGCG
TGCGCGATCTCGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4C332

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.138

100

0.498
  radA Streptococcus pneumoniae Rx1

46.855

100

0.472
  radA Streptococcus pneumoniae D39

46.855

100

0.472
  radA Streptococcus pneumoniae TIGR4

46.855

100

0.472
  radA Streptococcus pneumoniae R6

46.855

100

0.472
  radA Streptococcus mitis NCTC 12261

47.02

98.908

0.465
  radA Streptococcus mitis SK321

49.07

93.886

0.461

Multiple sequence alignment