Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DBK22_RS04290 Genome accession   NZ_CP028961
Coordinates   874588..875022 (+) Length   144 a.a.
NCBI ID   WP_053573457.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM102752     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 869588..880022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBK22_RS04285 (DBK22_00862) - 873088..874299 (-) 1212 WP_160241987.1 cytochrome P450 family protein -
  DBK22_RS04290 (DBK22_00863) nucA/comI 874588..875022 (+) 435 WP_053573457.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DBK22_RS04295 (DBK22_00864) - 875082..875837 (+) 756 WP_052827449.1 YoaK family protein -
  DBK22_RS04300 (DBK22_00865) - 875871..876233 (-) 363 WP_007410383.1 hypothetical protein -
  DBK22_RS04305 (DBK22_00866) - 876426..877754 (-) 1329 WP_012117603.1 S8 family peptidase -
  DBK22_RS04310 (DBK22_00867) - 877933..878166 (+) 234 WP_015239897.1 hypothetical protein -
  DBK22_RS04315 (DBK22_00868) - 878422..879129 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  DBK22_RS04320 (DBK22_00869) - 879189..879641 (+) 453 WP_039251441.1 OsmC family protein -
  DBK22_RS04325 (DBK22_00870) - 879655..880008 (-) 354 WP_007410379.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15548.46 Da        Isoelectric Point: 7.2418

>NTDB_id=288978 DBK22_RS04290 WP_053573457.1 874588..875022(+) (nucA/comI) [Bacillus velezensis strain SRCM102752]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=288978 DBK22_RS04290 WP_053573457.1 874588..875022(+) (nucA/comI) [Bacillus velezensis strain SRCM102752]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.829

81.25

0.486

Multiple sequence alignment