Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DBX26_RS13740 Genome accession   NZ_CP028943
Coordinates   2948804..2949820 (-) Length   338 a.a.
NCBI ID   WP_065302946.1    Uniprot ID   -
Organism   Vibrio sp. dhg     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2943804..2954820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBX26_RS13715 (DBX26_13690) aroK 2944676..2945194 (-) 519 WP_014233147.1 shikimate kinase AroK -
  DBX26_RS13720 (DBX26_13695) pilQ 2945383..2947122 (-) 1740 WP_162889729.1 type IV pilus secretin PilQ Machinery gene
  DBX26_RS13725 (DBX26_13700) pilP 2947154..2947669 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  DBX26_RS13730 (DBX26_13705) pilO 2947659..2948252 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  DBX26_RS13735 (DBX26_13710) pilN 2948245..2948820 (-) 576 WP_118119493.1 PilN domain-containing protein Machinery gene
  DBX26_RS13740 (DBX26_13715) pilM 2948804..2949820 (-) 1017 WP_065302946.1 type IV pilus assembly protein PilM Machinery gene
  DBX26_RS13745 (DBX26_13720) - 2949964..2952531 (+) 2568 WP_118119495.1 penicillin-binding protein 1A -
  DBX26_RS13750 (DBX26_13725) oxyR 2952594..2953502 (-) 909 WP_118119497.1 DNA-binding transcriptional regulator OxyR -
  DBX26_RS13755 (DBX26_13730) - 2953661..2954389 (+) 729 WP_014233155.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37641.83 Da        Isoelectric Point: 6.9713

>NTDB_id=288751 DBX26_RS13740 WP_065302946.1 2948804..2949820(-) (pilM) [Vibrio sp. dhg]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKNNYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIHCSVQESPLSGEEQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGRRALSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=288751 DBX26_RS13740 WP_065302946.1 2948804..2949820(-) (pilM) [Vibrio sp. dhg]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCGGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGCCCAGTTA
GCTACCAAGTTTTTGCGACACGTAAAGATGTCGTGGAGAGCCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTT
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAACAACTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAGTTTGCCT
GTGGAACAGGTTCCATTCATTGCTCTGTGCAGGAAAGCCCTCTGTCCGGAGAAGAGCAAGCCAAAACGAGTCAATTCAAT
CGTCAGATGGTCGATCGTGTCAAACGCCAGATACAGCTTTATACGTCCATTAACGGATCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTAGGAGGTCGCCGTGCTTTATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.852

100

0.749
  pilM Vibrio cholerae strain A1552

47.917

99.408

0.476

Multiple sequence alignment