Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CJ12664_RS05630 Genome accession   NZ_CP028912
Coordinates   1080542..1081501 (+) Length   319 a.a.
NCBI ID   WP_108614564.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NCTC 12664     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1060838..1093718 1080542..1081501 within 0


Gene organization within MGE regions


Location: 1060838..1093718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ12664_RS05540 (CJ12664_1069) pglI 1060838..1061767 (-) 930 WP_002890470.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CJ12664_RS05545 (CJ12664_1070) pglH 1061760..1062839 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CJ12664_RS05550 (CJ12664_1071) pglK 1062836..1064530 (-) 1695 WP_002890469.1 ABC-type lipopolysaccharide transporter PglK -
  CJ12664_RS05555 (CJ12664_1072) galE 1064524..1065510 (-) 987 WP_002853529.1 UDP-glucose 4-epimerase GalE -
  CJ12664_RS05560 (CJ12664_1073) - 1065568..1066362 (-) 795 WP_002853812.1 3'-5' exonuclease -
  CJ12664_RS05565 (CJ12664_1074) waaC 1066428..1067456 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  CJ12664_RS05570 (CJ12664_1075) htrB 1067446..1068333 (+) 888 WP_002890465.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CJ12664_RS05575 (CJ12664_1076) - 1068330..1069877 (+) 1548 WP_002858148.1 glycosyltransferase -
  CJ12664_RS05580 (CJ12664_1077) - 1069881..1071053 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  CJ12664_RS05585 (CJ12664_1078) - 1071037..1072032 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  CJ12664_RS05590 (CJ12664_1079) - 1072090..1073259 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  CJ12664_RS05595 (CJ12664_1080) wlaN 1073248..1074159 (-) 912 WP_002858039.1 beta-1,3 galactosyltransferase -
  CJ12664_RS05600 (CJ12664_1081) cstIII 1074214..1075098 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  CJ12664_RS05605 (CJ12664_1082) neuB1 1075079..1076110 (+) 1032 WP_002858213.1 sialic acid synthase -
  CJ12664_RS05610 (CJ12664_1083) neuC 1076107..1077222 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  CJ12664_RS08745 - 1077219..1078133 (+) 915 Protein_1079 beta-1,4-N-acetylgalactosaminyltransferase -
  CJ12664_RS08750 - 1078167..1078829 (+) 663 WP_002881429.1 N-acylneuraminate cytidylyltransferase -
  CJ12664_RS05620 (CJ12664_1085) - 1078824..1079675 (-) 852 WP_014516804.1 glycosyltransferase -
  CJ12664_RS05625 (CJ12664_1086) waaV 1079659..1080483 (-) 825 WP_108614563.1 glycosyltransferase family 2 protein -
  CJ12664_RS05630 (CJ12664_1087) waaF 1080542..1081501 (+) 960 WP_108614564.1 lipopolysaccharide heptosyltransferase II Regulator
  CJ12664_RS05635 (CJ12664_1088) gmhA 1081482..1082042 (-) 561 WP_002876196.1 D-sedoheptulose 7-phosphate isomerase -
  CJ12664_RS05640 (CJ12664_1089) rfaE1 1082039..1083424 (-) 1386 WP_002901184.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CJ12664_RS05645 (CJ12664_1090) rfaD 1083417..1084370 (-) 954 WP_002860242.1 ADP-glyceromanno-heptose 6-epimerase -
  CJ12664_RS05650 (CJ12664_1091) gmhB 1084371..1084932 (-) 562 Protein_1087 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CJ12664_RS05655 (CJ12664_1092) - 1085013..1085315 (+) 303 WP_002852762.1 cytochrome c -
  CJ12664_RS05660 (CJ12664_1093) ccoS 1085338..1085544 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  CJ12664_RS05665 (CJ12664_1094) - 1085541..1087898 (-) 2358 WP_079264979.1 heavy metal translocating P-type ATPase -
  CJ12664_RS05670 (CJ12664_1095) rho 1088007..1089305 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  CJ12664_RS05675 (CJ12664_1096) dnaX 1089309..1090838 (+) 1530 WP_032585175.1 DNA polymerase III subunit gamma/tau -
  CJ12664_RS08700 (CJ12664_1097) - 1090835..1091353 (-) 519 Protein_1093 DUF2165 family protein -
  CJ12664_RS05695 (CJ12664_1098) copA 1091425..1093524 (-) 2100 WP_108614565.1 copper-translocating P-type ATPase CopA -
  CJ12664_RS05700 (CJ12664_1099) - 1093524..1093718 (-) 195 WP_002902625.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36766.31 Da        Isoelectric Point: 10.2870

>NTDB_id=288482 CJ12664_RS05630 WP_108614564.1 1080542..1081501(+) (waaF) [Campylobacter jejuni strain NCTC 12664]
MKIFIHLPTWLGDAVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLGIKVHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAILVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHR

Nucleotide


Download         Length: 960 bp        

>NTDB_id=288482 CJ12664_RS05630 WP_108614564.1 1080542..1081501(+) (waaF) [Campylobacter jejuni strain NCTC 12664]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACGAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGGCATAAAAGTTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCACGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAACGCAATATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCGATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.597

99.687

0.953

Multiple sequence alignment