Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CJ12660_RS05920 Genome accession   NZ_CP028910
Coordinates   1122221..1123180 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NCTC 12660     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1102513..1132523 1122221..1123180 within 0


Gene organization within MGE regions


Location: 1102513..1132523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ12660_RS05830 (CJ12660_1124) pglI 1102513..1103442 (-) 930 WP_002852770.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CJ12660_RS05835 (CJ12660_1125) pglH 1103435..1104514 (-) 1080 WP_010891911.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CJ12660_RS05840 (CJ12660_1126) pglK 1104511..1106205 (-) 1695 WP_002858308.1 ABC-type lipopolysaccharide transporter PglK -
  CJ12660_RS05845 (CJ12660_1127) galE 1106199..1107185 (-) 987 WP_002858414.1 UDP-glucose 4-epimerase GalE -
  CJ12660_RS05850 (CJ12660_1128) - 1107243..1108037 (-) 795 WP_002853812.1 3'-5' exonuclease -
  CJ12660_RS05855 (CJ12660_1129) waaC 1108103..1109131 (+) 1029 WP_002858077.1 lipopolysaccharide heptosyltransferase I -
  CJ12660_RS05860 (CJ12660_1130) htrB 1109121..1110008 (+) 888 WP_002858051.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CJ12660_RS05865 (CJ12660_1131) - 1110005..1111552 (+) 1548 WP_002858148.1 glycosyltransferase -
  CJ12660_RS05870 (CJ12660_1132) - 1111556..1112728 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  CJ12660_RS05875 (CJ12660_1133) - 1112712..1113707 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  CJ12660_RS05880 (CJ12660_1134) - 1113765..1114934 (+) 1170 WP_002864814.1 glycosyltransferase family 2 protein -
  CJ12660_RS05885 (CJ12660_1135) wlaN 1114923..1115835 (-) 913 Protein_1137 beta-1,3 galactosyltransferase -
  CJ12660_RS05890 (CJ12660_1136) cstIII 1115890..1116774 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  CJ12660_RS05895 (CJ12660_1137) neuB1 1116755..1117786 (+) 1032 WP_002858213.1 sialic acid synthase -
  CJ12660_RS05900 (CJ12660_1138) neuC 1117783..1118898 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  CJ12660_RS09110 - 1118895..1119809 (+) 915 Protein_1141 beta-1,4-N-acetylgalactosaminyltransferase -
  CJ12660_RS09115 - 1119843..1120505 (+) 663 WP_002881429.1 N-acylneuraminate cytidylyltransferase -
  CJ12660_RS05910 (CJ12660_1140) - 1120500..1121354 (-) 855 WP_052806020.1 hypothetical protein -
  CJ12660_RS05915 (CJ12660_1141) waaV 1121338..1122162 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  CJ12660_RS05920 (CJ12660_1142) waaF 1122221..1123180 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  CJ12660_RS05925 (CJ12660_1143) gmhA 1123161..1123721 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  CJ12660_RS05930 (CJ12660_1144) rfaE1 1123718..1125103 (-) 1386 WP_052778309.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CJ12660_RS05935 (CJ12660_1145) rfaD 1125096..1126049 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  CJ12660_RS05940 (CJ12660_1146) gmhB 1126050..1126610 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CJ12660_RS05945 (CJ12660_1147) - 1126692..1126994 (+) 303 WP_002852762.1 cytochrome c -
  CJ12660_RS05950 (CJ12660_1148) ccoS 1127017..1127223 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  CJ12660_RS05955 (CJ12660_1149) - 1127220..1129577 (-) 2358 WP_075867355.1 heavy metal translocating P-type ATPase -
  CJ12660_RS05960 (CJ12660_1150) rho 1129686..1130984 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  CJ12660_RS05965 (CJ12660_1151) dnaX 1130988..1132523 (+) 1536 WP_002858347.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=288428 CJ12660_RS05920 WP_002875425.1 1122221..1123180(+) (waaF) [Campylobacter jejuni strain NCTC 12660]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=288428 CJ12660_RS05920 WP_002875425.1 1122221..1123180(+) (waaF) [Campylobacter jejuni strain NCTC 12660]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1

Multiple sequence alignment