Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DBX32_RS24515 Genome accession   NZ_CP028848
Coordinates   5146955..5147404 (-) Length   149 a.a.
NCBI ID   WP_031627735.1    Uniprot ID   A0A6N0KHJ6
Organism   Pseudomonas aeruginosa strain IMP67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5141955..5152404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBX32_RS24495 (DBX32_24425) ampD 5142635..5143201 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DBX32_RS24500 (DBX32_24430) - 5143352..5145607 (-) 2256 WP_034020466.1 DUF1631 domain-containing protein -
  DBX32_RS24505 (DBX32_24435) nadC 5145893..5146741 (+) 849 WP_023089629.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DBX32_RS24515 (DBX32_24445) pilA 5146955..5147404 (-) 450 WP_031627735.1 type 4a pilus biogenesis protein PilA Machinery gene
  DBX32_RS24520 (DBX32_24450) pilB 5147636..5149336 (+) 1701 WP_023089630.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DBX32_RS24525 (DBX32_24455) pilC 5149340..5150560 (+) 1221 WP_023089631.1 type 4a pilus biogenesis protein PilC Machinery gene
  DBX32_RS24530 (DBX32_24460) pilD 5150564..5151436 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  DBX32_RS24535 (DBX32_24465) coaE 5151433..5152044 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  DBX32_RS24540 (DBX32_24470) yacG 5152041..5152241 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15458.80 Da        Isoelectric Point: 8.4770

>NTDB_id=288095 DBX32_RS24515 WP_031627735.1 5146955..5147404(-) (pilA) [Pseudomonas aeruginosa strain IMP67]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVAPDANKLGVIAVAIEDSGAGDISFTFQTGTSSPKNATKAITLSRTADGVWTCKSTQDEMFIPKGCTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=288095 DBX32_RS24515 WP_031627735.1 5146955..5147404(-) (pilA) [Pseudomonas aeruginosa strain IMP67]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGCTCCGGATGCCAACAAGTTGGGTGTAATTGCTGTGGCAATCGAGGATAGTGGTGCAGGTGATATTTCCTTTAC
CTTCCAGACTGGTACCTCTAGTCCTAAGAACGCCACCAAGGCTATTACTCTGAGTCGTACTGCGGATGGTGTTTGGACTT
GTAAGTCCACTCAGGATGAGATGTTCATTCCGAAAGGCTGCACTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0KHJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.874

100

0.698
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.333

100

0.497
  pilA Vibrio cholerae strain A1552

49.333

100

0.497
  pilA Vibrio cholerae C6706

49.333

100

0.497
  pilA Acinetobacter baumannii strain A118

48.344

100

0.49
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.667

100

0.45
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA Vibrio parahaemolyticus RIMD 2210633

43.243

99.329

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.973

0.416
  pilA2 Legionella pneumophila str. Paris

37.931

97.315

0.369
  pilA2 Legionella pneumophila strain ERS1305867

37.931

97.315

0.369

Multiple sequence alignment