Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EGA47_RS02235 Genome accession   NZ_CP034106
Coordinates   490667..491335 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain RCAD0514     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 485667..496335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGA47_RS02205 (EGA47_02225) yicC 485687..486550 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  EGA47_RS02210 (EGA47_02230) rph 486677..487393 (+) 717 WP_001247089.1 ribonuclease PH -
  EGA47_RS02215 (EGA47_02235) pyrE 487459..488100 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EGA47_RS02220 (EGA47_02240) slmA 488137..488733 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EGA47_RS02225 (EGA47_02245) dut 488840..489298 (-) 459 WP_000976070.1 dUTP diphosphatase -
  EGA47_RS02230 (EGA47_02250) coaBC 489276..490495 (-) 1220 Protein_425 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EGA47_RS02235 (EGA47_02255) radC 490667..491335 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EGA47_RS02240 (EGA47_02260) rpmB 491552..491788 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  EGA47_RS02245 (EGA47_02265) rpmG 491809..491976 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  EGA47_RS02250 (EGA47_02270) mutM 492074..492883 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EGA47_RS02255 (EGA47_02275) coaD 492922..493401 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  EGA47_RS02260 (EGA47_02280) waaA 493409..494686 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EGA47_RS02265 (EGA47_02285) rfaQ 495099..496157 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=287893 EGA47_RS02235 WP_001297375.1 490667..491335(+) (radC) [Escherichia coli strain RCAD0514]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=287893 EGA47_RS02235 WP_001297375.1 490667..491335(+) (radC) [Escherichia coli strain RCAD0514]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496