Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG57_RS03080 Genome accession   NZ_CP028800
Coordinates   575796..576596 (+) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCHAJ59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570796..581596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG57_RS03055 (CDG57_03065) - 571706..573022 (+) 1317 WP_171133351.1 glycosyltransferase family 4 protein -
  CDG57_RS03060 (CDG57_03070) - 573050..573601 (+) 552 WP_087539381.1 phosphatase PAP2 family protein -
  CDG57_RS03065 (CDG57_03075) iscX 573684..573884 (+) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  CDG57_RS03070 (CDG57_03080) ndk 573991..574422 (+) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  CDG57_RS03075 (CDG57_03085) rlmN 574544..575779 (+) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG57_RS03080 (CDG57_03090) pilF 575796..576596 (+) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG57_RS03085 (CDG57_03095) - 576587..577378 (+) 792 WP_042891256.1 helix-turn-helix domain-containing protein -
  CDG57_RS03090 (CDG57_03100) ispG 577403..578518 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG57_RS03095 (CDG57_03105) hisS 578515..579807 (+) 1293 WP_087539383.1 histidine--tRNA ligase -
  CDG57_RS03100 (CDG57_03110) - 579839..580537 (+) 699 WP_087539385.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=287798 CDG57_RS03080 WP_004908991.1 575796..576596(+) (pilF) [Acinetobacter junii strain WCHAJ59]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=287798 CDG57_RS03080 WP_004908991.1 575796..576596(+) (pilF) [Acinetobacter junii strain WCHAJ59]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment