Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EHS18_RS08135 Genome accession   NZ_CP034098
Coordinates   1651162..1653435 (-) Length   757 a.a.
NCBI ID   WP_000595001.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 80wphwpl_v1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1646162..1658435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHS18_RS08115 (EHS18_08115) - 1646669..1647544 (-) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -
  EHS18_RS08120 (EHS18_08120) dtd 1647541..1647993 (-) 453 WP_124733822.1 D-aminoacyl-tRNA deacylase -
  EHS18_RS08125 (EHS18_08125) - 1648005..1650194 (-) 2190 WP_001058583.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  EHS18_RS08130 (EHS18_08130) - 1650622..1651140 (-) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  EHS18_RS08135 (EHS18_08135) recJ 1651162..1653435 (-) 2274 WP_000595001.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EHS18_RS08140 (EHS18_08140) secDF 1653638..1655917 (-) 2280 WP_000749802.1 protein translocase subunit SecDF -
  EHS18_RS08145 (EHS18_08145) - 1656168..1657814 (-) 1647 WP_000277741.1 IS1182-like element ISSau3 family transposase -
  EHS18_RS08150 (EHS18_08150) yajC 1658113..1658373 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85592.81 Da        Isoelectric Point: 4.8064

>NTDB_id=287755 EHS18_RS08135 WP_000595001.1 1651162..1653435(-) (recJ) [Staphylococcus aureus strain 80wphwpl_v1]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEQFNRERK
DIVATITEEAMAMAEMKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=287755 EHS18_RS08135 WP_000595001.1 1651162..1653435(-) (recJ) [Staphylococcus aureus strain 80wphwpl_v1]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATTGAATCCATCATTTCAGATACAG
ATATCAATCATGACGCATTGCAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAGCTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCGTACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCATTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACAATTTAACCGCGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCAATGGCAGAAATGAAAGTTAAAAATGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACACCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCCAGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTGAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAATTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAC
GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCTACGTCAATGGAAGATTTGTCTAATTCCCTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATGTTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

RecJ OB domain (RecJ_OB)

(452-556)

DHHA1 domain (DHHA1)

(312-436)

DHH family (DHH)

(81-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.144

100

0.391
  recJ Streptococcus pneumoniae strain D39V

38.451

100

0.387

Multiple sequence alignment