Detailed information NTG0028680

Bioinformatically predicted

Overview

• Name: radC
• Type: Machinery gene
• Locus tag: ECH74115_RS24860
• Genome accession: NC_011353
• Coordinates: 4658854..4659522 (-)
• Length: 222 a.a.
• NCBI ID: WP_001297375.1
• Uniprot ID: P65957
• Organism: Escherichia coli O157:H7 str. EC4115
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4653854..4664522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECH74115_RS24830 (ECH74115_5002)	rfaQ	4654032..4655090 (-)	1059	WP_000360402.1	lipopolysaccharide core heptosyltransferase RfaQ	-
ECH74115_RS24835 (ECH74115_5003)	waaA	4655503..4656780 (+)	1278	WP_000891564.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
ECH74115_RS24840 (ECH74115_5004)	coaD	4656788..4657267 (+)	480	WP_001171866.1	pantetheine-phosphate adenylyltransferase	-
ECH74115_RS24845 (ECH74115_5005)	mutM	4657306..4658115 (-)	810	WP_001114533.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
ECH74115_RS24850 (ECH74115_5006)	rpmG	4658213..4658380 (-)	168	WP_001051798.1	50S ribosomal protein L33	-
ECH74115_RS24855 (ECH74115_5007)	rpmB	4658401..4658637 (-)	237	WP_000091955.1	50S ribosomal protein L28	-
ECH74115_RS24860 (ECH74115_5008)	radC	4658854..4659522 (-)	669	WP_001297375.1	DNA repair protein RadC	Machinery gene
ECH74115_RS24865 (ECH74115_5009)	coaBC	4659694..4660914 (+)	1221	WP_000050139.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ECH74115_RS24870 (ECH74115_5010)	dut	4660892..4661350 (+)	459	WP_000976070.1	dUTP diphosphatase	-
ECH74115_RS24875 (ECH74115_5011)	slmA	4661457..4662053 (+)	597	WP_000818601.1	nucleoid occlusion factor SlmA	-
ECH74115_RS24880 (ECH74115_5012)	pyrE	4662090..4662731 (-)	642	WP_000806161.1	orotate phosphoribosyltransferase	-
ECH74115_RS24885 (ECH74115_5013)	rph	4662797..4663513 (-)	717	WP_001247093.1	ribonuclease PH	-
ECH74115_RS24890 (ECH74115_5014)	yicC	4663640..4664503 (+)	864	WP_000621336.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 222 a.a.
• Molecular weight: 25258.35 Da
• Isoelectric Point: 7.9748

>NTDB_id=28680 ECH74115_RS24860 WP_001297375.1 4658854..4659522(-) (radC) [Escherichia coli O157:H7 str. EC4115]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide

• Length: 669 bp

>NTDB_id=28680 ECH74115_RS24860 WP_001297375.1 4658854..4659522(-) (radC) [Escherichia coli O157:H7 str. EC4115]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

• RadC-like JAB domain (RadC) (103-220)

• UPF0758 N-terminal (UPF0758_N) (9-77)

3D structure

• AlphaFold DB: P65957

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radC	Haemophilus influenzae Rd KW20	49.55	100	0.496