Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EGM66_RS14565 Genome accession   NZ_CP033884
Coordinates   2545659..2546135 (-) Length   158 a.a.
NCBI ID   WP_001186756.1    Uniprot ID   A0A0H2VD88
Organism   Escherichia coli strain 50579417     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2540659..2551135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM66_RS14525 (EGM66_14525) istA 2540700..2542199 (+) 1500 WP_029700729.1 IS21-like element ISEc10 family transposase -
  EGM66_RS14530 (EGM66_14530) istB 2542196..2542951 (+) 756 WP_000065240.1 IS21-like element ISEc10 family helper ATPase IstB -
  EGM66_RS14535 (EGM66_14535) - 2543000..2543710 (-) 711 WP_001621817.1 DUF4942 domain-containing protein -
  EGM66_RS14540 (EGM66_14540) - 2543795..2543992 (-) 198 WP_000839254.1 DUF957 domain-containing protein -
  EGM66_RS14545 (EGM66_14545) - 2544004..2544492 (-) 489 WP_000761714.1 DUF5983 family protein -
  EGM66_RS14550 (EGM66_14550) - 2544489..2544866 (-) 378 WP_001094436.1 TA system toxin CbtA family protein -
  EGM66_RS14555 (EGM66_14555) cbeA 2544913..2545290 (-) 378 WP_015953067.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA -
  EGM66_RS14560 (EGM66_14560) - 2545369..2545590 (-) 222 WP_000692312.1 DUF987 domain-containing protein -
  EGM66_RS14565 (EGM66_14565) radC 2545659..2546135 (-) 477 WP_001186756.1 RadC family protein Machinery gene
  EGM66_RS14570 (EGM66_14570) - 2546150..2546635 (-) 486 WP_000860054.1 antirestriction protein -
  EGM66_RS14575 (EGM66_14575) - 2546726..2547544 (-) 819 WP_001175142.1 DUF932 domain-containing protein -
  EGM66_RS14580 (EGM66_14580) - 2547634..2547867 (-) 234 WP_001278287.1 DUF905 family protein -
  EGM66_RS14585 (EGM66_14585) - 2547873..2548550 (-) 678 WP_001097312.1 hypothetical protein -
  EGM66_RS14590 (EGM66_14590) - 2548698..2549378 (-) 681 WP_001282927.1 WYL domain-containing protein -
  EGM66_RS14595 (EGM66_14595) - 2549581..2550465 (-) 885 WP_000010416.1 GTPase -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 17661.31 Da        Isoelectric Point: 7.6149

>NTDB_id=285748 EGM66_RS14565 WP_001186756.1 2545659..2546135(-) (radC) [Escherichia coli strain 50579417]
MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAREWLILNMAGLEREEFRVLYLNNQNKLIAGETLFTGT
INRTEVHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=285748 EGM66_RS14565 WP_001186756.1 2545659..2546135(-) (radC) [Escherichia coli strain 50579417]
ATGCAACAGCTTTCCTTTCTGCCAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAGCGGGCCCTGAAAACCCTGGA
CCGCCATCTTCATGAACCCGGTGTGGCCTTCACCTCCACCCGTGCGGCACGGGAATGGCTGATTCTGAACATGGCGGGAC
TGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATAAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACC
ATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGTGGTACTGGCGCA
CAATCACCCGTCCGGTGAAGTCACACCCAGCAAGGCAGACCGCCTTATCACGGAACGTCTGGTACAGGCACTGGGCCTGG
TGGATATCCGGGTGCCGGACCATCTGATAGTCGGTGGCAGCCAGGTTTTCTCCTTTGCGGAACACGGTCTGCTTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(38-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VD88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

55.752

71.519

0.399