Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EGM66_RS02595 Genome accession   NZ_CP033884
Coordinates   53287..54714 (+) Length   475 a.a.
NCBI ID   WP_021523141.1    Uniprot ID   -
Organism   Escherichia coli strain 50579417     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48287..59714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM66_RS02555 (EGM66_02565) yeeW 48471..48665 (+) 195 WP_000988600.1 DUF5983 family protein -
  EGM66_RS28075 (EGM66_02570) - 48711..48791 (+) 81 Protein_48 hypothetical protein -
  EGM66_RS28740 (EGM66_02580) - 49280..49462 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  EGM66_RS02575 (EGM66_02585) tmaR 49563..49892 (-) 330 WP_000450409.1 DUF496 family protein -
  EGM66_RS02580 (EGM66_02590) yeeA 50064..51122 (-) 1059 WP_001200890.1 FUSC family protein -
  EGM66_RS02585 (EGM66_02595) sbmC 51320..51793 (-) 474 WP_001105416.1 DNA gyrase inhibitor SbmC -
  EGM66_RS02590 (EGM66_02600) dacD 51912..53078 (-) 1167 WP_021523140.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EGM66_RS02595 (EGM66_02605) sbcB 53287..54714 (+) 1428 WP_021523141.1 exodeoxyribonuclease I Machinery gene
  EGM66_RS02600 (EGM66_02610) tsuB 54757..54984 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EGM66_RS02605 (EGM66_02615) tsuA 54998..56056 (-) 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
  EGM66_RS02610 (EGM66_02620) plaP 56235..57593 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  EGM66_RS02615 (EGM66_02625) yoeI 57583..57645 (-) 63 WP_010723108.1 membrane protein YoeI -
  EGM66_RS02620 (EGM66_02630) yeeY 57860..58789 (-) 930 WP_023154044.1 LysR substrate-binding domain-containing protein -
  EGM66_RS02625 (EGM66_02635) yeeZ 58835..59659 (-) 825 WP_000754758.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54513.81 Da        Isoelectric Point: 5.4700

>NTDB_id=285694 EGM66_RS02595 WP_021523141.1 53287..54714(+) (sbcB) [Escherichia coli strain 50579417]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNVVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=285694 EGM66_RS02595 WP_021523141.1 53287..54714(+) (sbcB) [Escherichia coli strain 50579417]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTAATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGTCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGTCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCACATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581