Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG55_RS12090 Genome accession   NZ_CP028561
Coordinates   2228010..2228813 (-) Length   267 a.a.
NCBI ID   WP_087535641.1    Uniprot ID   A0A2R4TLW2
Organism   Acinetobacter sp. WCHA45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2223010..2233813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS12070 (CDG55_12065) - 2224051..2224749 (-) 699 WP_087535645.1 YfgM family protein -
  CDG55_RS12075 (CDG55_12070) hisS 2224781..2226073 (-) 1293 WP_087535644.1 histidine--tRNA ligase -
  CDG55_RS12080 (CDG55_12075) ispG 2226081..2227196 (-) 1116 WP_087535643.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG55_RS12085 (CDG55_12080) - 2227219..2228019 (-) 801 WP_087535642.1 helix-turn-helix domain-containing protein -
  CDG55_RS12090 (CDG55_12085) pilF 2228010..2228813 (-) 804 WP_087535641.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG55_RS12095 (CDG55_12090) rlmN 2228831..2230066 (-) 1236 WP_087535640.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG55_RS12100 (CDG55_12095) ndk 2230188..2230619 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  CDG55_RS12105 (CDG55_12100) iscX 2230728..2230928 (-) 201 WP_004664895.1 Fe-S cluster assembly protein IscX -
  CDG55_RS12110 (CDG55_12105) - 2231023..2231574 (-) 552 WP_087535639.1 phosphatase PAP2 family protein -
  CDG55_RS12115 (CDG55_12110) - 2231599..2232885 (-) 1287 WP_087535638.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30267.27 Da        Isoelectric Point: 9.4465

>NTDB_id=285520 CDG55_RS12090 WP_087535641.1 2228010..2228813(-) (pilF) [Acinetobacter sp. WCHA45]
MNTTRLKTIITTTVVFTVMGLTACQSSTGLKKDPEKAVQVRSQLAAEYIRTGDLDSAKRSLDQALNIDSRDANANMMMGI
LLQQEGSKPNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYEQRFQSLENLGRIYLKLGD
VANAEKAFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARASADQLGMQVLVN
QLRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=285520 CDG55_RS12090 WP_087535641.1 2228010..2228813(-) (pilF) [Acinetobacter sp. WCHA45]
TTGAATACAACTCGACTAAAAACGATTATAACGACTACTGTTGTGTTTACTGTAATGGGTTTAACCGCTTGTCAATCTTC
GACAGGGTTGAAGAAAGACCCAGAAAAGGCTGTTCAAGTTCGTTCTCAACTGGCTGCTGAATATATTCGTACAGGTGATT
TGGATTCCGCTAAACGTTCTCTTGATCAAGCTTTAAATATTGATTCGCGTGATGCAAACGCAAATATGATGATGGGGATT
CTACTGCAACAAGAAGGCAGTAAACCTAATATGGAAAAAGCAGATGCTTATTTTAAGCGTGCGATTTCTTCAGAACCAGA
TAATGCACAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAAGCGATTGAGCAATTTACCC
GTGCAGGTGCAACCTTAGGTTATGAACAGCGATTTCAATCGTTGGAAAACTTGGGGCGAATCTATTTAAAGTTAGGCGAT
GTTGCGAATGCTGAAAAAGCATTCAAACAAGCGCTACAAGCAAATCGTAATGCAACTATTTCAATGTTAGAGTTGGCAGA
AATTTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTATACGAACAGTATGTTCGTAATGTGGGGCAAAAAAACC
AAGGTGCACGTGCACTTTGGATTGGTTTACGCATTGCTCGAGCGAGTGCGGATCAATTAGGAATGCAAGTGTTAGTGAAT
CAATTACGAGCATTATTTCCTGAGAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
ATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(43-69)

Tetratricopeptide repeat (TPR_12)

(109-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R4TLW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.79
  pilF Acinetobacter baylyi ADP1

67.816

97.753

0.663

Multiple sequence alignment