Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EGY17_RS13840 Genome accession   NZ_CP033850
Coordinates   2475645..2476085 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain FDAARGOS_497     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2470645..2481085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY17_RS13820 (EGY17_13840) coaE 2471135..2471755 (-) 621 WP_022645176.1 dephospho-CoA kinase -
  EGY17_RS27560 - 2471780..2471824 (+) 45 WP_120795372.1 protein YacM -
  EGY17_RS13825 (EGY17_13845) guaC 2471980..2473023 (+) 1044 WP_001217337.1 GMP reductase -
  EGY17_RS13830 (EGY17_13850) hofC 2473058..2474260 (-) 1203 WP_022645177.1 protein transport protein HofC -
  EGY17_RS13835 (EGY17_13855) pilB 2474250..2475635 (-) 1386 WP_022645178.1 type II secretion system protein GspE Machinery gene
  EGY17_RS13840 (EGY17_13860) pilA 2475645..2476085 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  EGY17_RS13845 (EGY17_13865) nadC 2476288..2477181 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGY17_RS13850 (EGY17_13870) ampD 2477269..2477820 (+) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EGY17_RS13855 (EGY17_13875) ampE 2477817..2478671 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  EGY17_RS13860 (EGY17_13880) aroP 2478714..2480087 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=285424 EGY17_RS13840 WP_000360914.1 2475645..2476085(-) (pilA) [Escherichia coli strain FDAARGOS_497]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=285424 EGY17_RS13840 WP_000360914.1 2475645..2476085(-) (pilA) [Escherichia coli strain FDAARGOS_497]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363