Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DBP88_RS21265 Genome accession   NZ_CP028538
Coordinates   3937435..3938421 (-) Length   328 a.a.
NCBI ID   WP_196355302.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain SCEH020042     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3932435..3943421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBP88_RS21235 (DBP88_21360) - 3933312..3933809 (+) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -
  DBP88_RS21240 (DBP88_21365) endA 3933904..3934611 (+) 708 WP_023306912.1 deoxyribonuclease I -
  DBP88_RS21245 (DBP88_21370) rsmE 3934663..3935394 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DBP88_RS21250 (DBP88_21375) gshB 3935426..3936373 (+) 948 WP_023306913.1 glutathione synthase -
  DBP88_RS21255 (DBP88_21380) - 3936448..3937008 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  DBP88_RS21260 (DBP88_21385) ruvX 3937008..3937424 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  DBP88_RS21265 (DBP88_21390) pilT 3937435..3938421 (-) 987 WP_196355302.1 type IV pilus twitching motility protein PilT Machinery gene
  DBP88_RS21270 (DBP88_21395) - 3938433..3939134 (+) 702 WP_023298569.1 YggS family pyridoxal phosphate-dependent enzyme -
  DBP88_RS21275 (DBP88_21400) - 3939156..3939722 (+) 567 WP_003860024.1 YggT family protein -
  DBP88_RS21280 (DBP88_21405) yggU 3939719..3940015 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  DBP88_RS21285 (DBP88_21410) - 3940019..3940612 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  DBP88_RS21290 (DBP88_21415) hemW 3940605..3941753 (+) 1149 WP_023306915.1 radical SAM family heme chaperone HemW -
  DBP88_RS21295 (DBP88_21420) - 3941810..3942172 (+) 363 WP_108168740.1 endonuclease domain-containing protein -
  DBP88_RS21300 (DBP88_21425) - 3942240..3942956 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  DBP88_RS21305 (DBP88_21430) - 3943014..3943340 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 328 a.a.        Molecular weight: 35888.91 Da        Isoelectric Point: 6.8304

>NTDB_id=285420 DBP88_RS21265 WP_196355302.1 3937435..3938421(-) (pilT) [Enterobacter hormaechei strain SCEH020042]
MSKDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRL
RGSAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDP
VEFMYQSQRCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLV
DTFPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLA
ERRAQGRL

Nucleotide


Download         Length: 987 bp        

>NTDB_id=285420 DBP88_RS21265 WP_196355302.1 3937435..3938421(-) (pilT) [Enterobacter hormaechei strain SCEH020042]
TTGTCTAAGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACC
ACCGCGCTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGC
TCAATGACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTG
CGCGGCAGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGC
GCTAGGCGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCG
GGAAATCGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCG
GTGGAGTTTATGTACCAGAGCCAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGC
CCTGCGCAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGC
TGACGGCGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTC
GATACCTTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCT
GCGTCAGGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCG
AAGGTAAAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCG
GAGCGACGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.613

99.39

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.613

99.39

0.503
  pilT Pseudomonas stutzeri DSM 10701

47.853

99.39

0.476
  pilT Legionella pneumophila strain Lp02

48.287

97.866

0.473
  pilT Legionella pneumophila strain ERS1305867

48.287

97.866

0.473
  pilT Neisseria meningitidis 8013

47.546

99.39

0.473
  pilT Neisseria gonorrhoeae MS11

47.239

99.39

0.47
  pilT Acinetobacter baumannii strain A118

46.933

99.39

0.466
  pilT Acinetobacter baylyi ADP1

46.933

99.39

0.466
  pilT Acinetobacter baumannii D1279779

46.933

99.39

0.466
  pilT Pseudomonas aeruginosa PAK

46.933

99.39

0.466
  pilT Acinetobacter nosocomialis M2

47.335

97.256

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.171

0.427
  pilU Pseudomonas stutzeri DSM 10701

38.298

100

0.384
  pilU Vibrio cholerae strain A1552

39.677

94.512

0.375

Multiple sequence alignment