Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DA379_RS15510 Genome accession   NZ_CP028441
Coordinates   3114794..3115939 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain 131-4     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3109794..3120939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA379_RS15490 - 3110268..3110984 (-) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -
  DA379_RS15495 narK 3111082..3112272 (-) 1191 WP_003151073.1 nitrate transporter NarK -
  DA379_RS15500 argS 3112407..3114077 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  DA379_RS15505 - 3114074..3114502 (-) 429 WP_003151069.1 DUF1934 family protein -
  DA379_RS15510 rapF 3114794..3115939 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  DA379_RS15515 - 3115923..3116042 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  DA379_RS15520 - 3116241..3116444 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  DA379_RS15525 - 3116458..3116661 (+) 204 WP_003151062.1 hypothetical protein -
  DA379_RS19825 - 3116696..3116881 (+) 186 Protein_3022 DUF4177 domain-containing protein -
  DA379_RS15535 - 3117069..3117350 (+) 282 WP_003151060.1 hypothetical protein -
  DA379_RS15540 - 3117394..3117906 (+) 513 WP_003151059.1 stage II sporulation protein M -
  DA379_RS15550 - 3118497..3118715 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  DA379_RS15555 speB 3118885..3119757 (-) 873 WP_003151054.1 agmatinase -
  DA379_RS15560 speE 3119817..3120647 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=285107 DA379_RS15510 WP_003151067.1 3114794..3115939(+) (rapF) [Bacillus velezensis strain 131-4]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=285107 DA379_RS15510 WP_003151067.1 3114794..3115939(+) (rapF) [Bacillus velezensis strain 131-4]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53

Multiple sequence alignment