Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DA378_RS13355 Genome accession   NZ_CP028440
Coordinates   2729587..2730732 (-) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain J7-1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2724587..2735732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA378_RS13305 speE 2724879..2725709 (+) 831 WP_003151052.1 spermidine synthase -
  DA378_RS13310 speB 2725769..2726641 (+) 873 WP_003151054.1 agmatinase -
  DA378_RS13315 - 2726811..2727029 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  DA378_RS13325 - 2727620..2728132 (-) 513 WP_003151059.1 stage II sporulation protein M -
  DA378_RS13330 - 2728176..2728457 (-) 282 WP_003151060.1 hypothetical protein -
  DA378_RS19850 - 2728645..2728830 (-) 186 Protein_2564 DUF4177 domain-containing protein -
  DA378_RS13340 - 2728865..2729068 (-) 204 WP_003151062.1 hypothetical protein -
  DA378_RS13345 - 2729082..2729285 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  DA378_RS13350 - 2729484..2729603 (-) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  DA378_RS13355 rapF 2729587..2730732 (-) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  DA378_RS13360 - 2731024..2731452 (+) 429 WP_003151069.1 DUF1934 family protein -
  DA378_RS13365 argS 2731449..2733119 (+) 1671 WP_003151071.1 arginine--tRNA ligase -
  DA378_RS13370 narK 2733254..2734444 (+) 1191 WP_003151073.1 nitrate transporter NarK -
  DA378_RS13375 - 2734542..2735258 (+) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=285010 DA378_RS13355 WP_003151067.1 2729587..2730732(-) (rapF) [Bacillus velezensis strain J7-1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=285010 DA378_RS13355 WP_003151067.1 2729587..2730732(-) (rapF) [Bacillus velezensis strain J7-1]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53

Multiple sequence alignment