Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DA378_RS09815 Genome accession   NZ_CP028440
Coordinates   2062886..2063323 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain J7-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2057886..2068323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA378_RS09800 - 2060744..2061106 (-) 363 WP_003156585.1 helix-turn-helix domain-containing protein -
  DA378_RS09805 hxlA 2061338..2061973 (+) 636 WP_003156586.1 3-hexulose-6-phosphate synthase -
  DA378_RS09810 hxlB 2061970..2062527 (+) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  DA378_RS09815 nucA/comI 2062886..2063323 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DA378_RS09820 nin/comJ 2063345..2063743 (+) 399 WP_003156589.1 competence protein ComJ Regulator
  DA378_RS09825 - 2063784..2064002 (-) 219 Protein_1920 family 1 glycosylhydrolase -
  DA378_RS19995 - 2064001..2064279 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  DA378_RS09835 - 2064221..2064541 (-) 321 WP_003156590.1 YckD family protein -
  DA378_RS09840 - 2064613..2065047 (-) 435 WP_003156592.1 RDD family protein -
  DA378_RS09845 - 2065062..2066198 (-) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  DA378_RS09850 - 2066263..2067465 (-) 1203 WP_003156595.1 GTP-binding protein -
  DA378_RS19820 - 2067604..2067740 (+) 137 Protein_1926 molybdopterin dinucleotide binding domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=284999 DA378_RS09815 WP_003156588.1 2062886..2063323(+) (nucA/comI) [Bacillus velezensis strain J7-1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=284999 DA378_RS09815 WP_003156588.1 2062886..2063323(+) (nucA/comI) [Bacillus velezensis strain J7-1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment