Detailed information NTG0284982

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EGY23_RS03520
• Genome accession: NZ_CP033832
• Coordinates: 752816..753880 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain FDAARGOS_505
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 747816..758880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGY23_RS03495 (EGY23_03485)	aroK	748351..748869 (-)	519	WP_123789195.1	shikimate kinase AroK	-
EGY23_RS03500 (EGY23_03490)	pilQ	748881..751025 (-)	2145	WP_023083249.1	type 4a pilus secretin PilQ	Machinery gene
EGY23_RS03505 (EGY23_03495)	comL/pilP	751079..751603 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
EGY23_RS03510 (EGY23_03500)	pilO	751600..752223 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
EGY23_RS03515 (EGY23_03505)	pilN	752220..752816 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
EGY23_RS03520 (EGY23_03510)	pilM	752816..753880 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
EGY23_RS03525 (EGY23_03515)	-	754065..756536 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
EGY23_RS03530 (EGY23_03520)	-	756641..757909 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=284982 EGY23_RS03520 WP_003110888.1 752816..753880(-) (pilM) [Pseudomonas aeruginosa strain FDAARGOS_505]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=284982 EGY23_RS03520 WP_003110888.1 752816..753880(-) (pilM) [Pseudomonas aeruginosa strain FDAARGOS_505]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466