Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   DA377_RS08890 Genome accession   NZ_CP028439
Coordinates   1671697..1672497 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain 8-2     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 1666697..1677497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA377_RS08870 - 1666821..1667666 (+) 846 WP_003153172.1 TlyA family RNA methyltransferase -
  DA377_RS08875 argR 1667761..1668210 (+) 450 WP_003153173.1 transcriptional regulator ArgR -
  DA377_RS08880 recN 1668242..1669972 (+) 1731 WP_003153174.1 DNA repair protein RecN Machinery gene
  DA377_RS08885 spoIVB 1670136..1671416 (+) 1281 WP_003153176.1 SpoIVB peptidase -
  DA377_RS08890 spo0A 1671697..1672497 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  DA377_RS08895 alr 1672645..1673826 (-) 1182 WP_003153179.1 alanine racemase -
  DA377_RS08900 - 1674002..1674214 (-) 213 WP_003153181.1 spore germination protein -
  DA377_RS08905 - 1674340..1674552 (-) 213 WP_003153183.1 spore germination protein -
  DA377_RS08910 - 1674679..1675797 (-) 1119 WP_003153184.1 NADH-dependent flavin oxidoreductase -
  DA377_RS19610 - 1675891..1676028 (-) 138 WP_014470646.1 hypothetical protein -
  DA377_RS08915 - 1676319..1676951 (-) 633 WP_003153189.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=284936 DA377_RS08890 WP_003153177.1 1671697..1672497(+) (spo0A) [Bacillus velezensis strain 8-2]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=284936 DA377_RS08890 WP_003153177.1 1671697..1672497(+) (spo0A) [Bacillus velezensis strain 8-2]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGTG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGGTATACCGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment