Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   DA377_RS08385 Genome accession   NZ_CP028439
Coordinates   1586462..1587229 (+) Length   255 a.a.
NCBI ID   WP_003152982.1    Uniprot ID   -
Organism   Bacillus velezensis strain 8-2     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1581462..1592229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA377_RS08355 - 1581745..1583877 (+) 2133 WP_003152972.1 HD family phosphohydrolase -
  DA377_RS08360 ybeY 1583878..1584351 (+) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  DA377_RS08365 - 1584332..1584706 (+) 375 WP_003152974.1 diacylglycerol kinase family protein -
  DA377_RS08370 - 1584830..1585240 (+) 411 WP_003152976.1 cytidine deaminase -
  DA377_RS08375 era 1585221..1586126 (+) 906 WP_003152978.1 GTPase Era -
  DA377_RS08380 - 1586282..1586425 (+) 144 WP_003152980.1 YqzL family protein -
  DA377_RS08385 recO 1586462..1587229 (+) 768 WP_003152982.1 DNA repair protein RecO Machinery gene
  DA377_RS08390 glyQ 1587531..1588418 (+) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  DA377_RS08395 glyS 1588411..1590450 (+) 2040 WP_003152986.1 glycine--tRNA ligase subunit beta -
  DA377_RS08400 ccpN 1590551..1591189 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  DA377_RS08405 - 1591215..1592027 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29264.86 Da        Isoelectric Point: 8.3099

>NTDB_id=284927 DA377_RS08385 WP_003152982.1 1586462..1587229(+) (recO) [Bacillus velezensis strain 8-2]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKQVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=284927 DA377_RS08385 WP_003152982.1 1586462..1587229(+) (recO) [Bacillus velezensis strain 8-2]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACATTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACAGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.412

100

0.894

Multiple sequence alignment