Detailed information NTG0284893

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: DA377_RS00475
• Genome accession: NZ_CP028439
• Coordinates: 75151..76530 (-)
• Length: 459 a.a.
• NCBI ID: WP_003156400.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 8-2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 70151..81530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA377_RS00455	ispF	71671..72147 (-)	477	WP_003156407.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
DA377_RS00460	ispD	72140..72838 (-)	699	WP_003156405.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
DA377_RS00465	-	72851..73951 (-)	1101	WP_003156403.1	PIN/TRAM domain-containing protein	-
DA377_RS00470	disA	74065..75147 (-)	1083	WP_003156401.1	DNA integrity scanning diadenylate cyclase DisA	-
DA377_RS00475	radA	75151..76530 (-)	1380	WP_003156400.1	DNA repair protein RadA	Machinery gene
DA377_RS00480	clpC	76624..79059 (-)	2436	WP_003156399.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
DA377_RS00485	-	79056..80147 (-)	1092	WP_003156398.1	protein arginine kinase	-
DA377_RS00490	-	80147..80704 (-)	558	WP_003156397.1	UvrB/UvrC motif-containing protein	-
DA377_RS00495	ctsR	80718..81182 (-)	465	WP_003156396.1	transcriptional regulator CtsR	-

Sequence

Protein

• Length: 459 a.a.
• Molecular weight: 49553.04 Da
• Isoelectric Point: 7.4033

>NTDB_id=284893 DA377_RS00475 WP_003156400.1 75151..76530(-) (radA) [Bacillus velezensis strain 8-2]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGIKSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide

• Length: 1380 bp

>NTDB_id=284893 DA377_RS00475 WP_003156400.1 75151..76530(-) (radA) [Bacillus velezensis strain 8-2]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTGATCGGCGGCGATCCCGGGATCGGAAAATCCACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGCATCAAAAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCATCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTCAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCATATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (71-222)

• Subunit ChlI of Mg-chelatase (ChlI) (364-430)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	93.231	99.782	0.93
radA	Streptococcus pneumoniae Rx1	63.797	98.693	0.63
radA	Streptococcus pneumoniae D39	63.797	98.693	0.63
radA	Streptococcus pneumoniae R6	63.797	98.693	0.63
radA	Streptococcus pneumoniae TIGR4	63.797	98.693	0.63
radA	Streptococcus mitis NCTC 12261	63.797	98.693	0.63
radA	Streptococcus mitis SK321	63.576	98.693	0.627