Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DA376_RS12320 Genome accession   NZ_CP028437
Coordinates   2536648..2537262 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain DR-08     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531648..2542262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA376_RS12295 (DA376_12295) rpsT 2531951..2532217 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  DA376_RS12300 (DA376_12300) holA 2532234..2533275 (-) 1042 Protein_2359 DNA polymerase III subunit delta -
  DA376_RS19660 - 2533315..2533466 (-) 152 Protein_2360 hypothetical protein -
  DA376_RS12305 (DA376_12305) - 2533507..2533641 (+) 135 WP_003152870.1 YqzM family protein -
  DA376_RS12310 (DA376_12310) comEC 2533660..2536011 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DA376_RS12315 (DA376_12315) - 2536012..2536581 (-) 570 WP_003152868.1 ComE operon protein 2 -
  DA376_RS12320 (DA376_12320) comEA 2536648..2537262 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  DA376_RS12325 (DA376_12325) comER 2537321..2538142 (+) 822 WP_012118027.1 late competence protein ComER -
  DA376_RS12330 (DA376_12330) - 2538211..2538948 (-) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  DA376_RS12335 (DA376_12335) rsfS 2538945..2539301 (-) 357 WP_007408260.1 ribosome silencing factor -
  DA376_RS12340 (DA376_12340) yqeK 2539305..2539880 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DA376_RS12345 (DA376_12345) - 2539870..2540439 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  DA376_RS12350 (DA376_12350) yhbY 2540450..2540740 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  DA376_RS12355 (DA376_12355) aroE 2540734..2541576 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=284869 DA376_RS12320 WP_032873955.1 2536648..2537262(-) (comEA) [Bacillus velezensis strain DR-08]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=284869 DA376_RS12320 WP_032873955.1 2536648..2537262(-) (comEA) [Bacillus velezensis strain DR-08]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment