Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SK637_RS01630 Genome accession   NZ_CP028415
Coordinates   310489..311313 (+) Length   274 a.a.
NCBI ID   WP_033688119.1    Uniprot ID   -
Organism   Streptococcus mitis strain SK637     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 305489..316313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK637_RS01595 (SK637_00313) asnA 305580..306572 (+) 993 WP_033688114.1 aspartate--ammonia ligase -
  SK637_RS01600 (SK637_00314) rsmD 306637..307176 (+) 540 WP_004260538.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SK637_RS01605 (SK637_00315) coaD 307166..307654 (+) 489 WP_033688115.1 pantetheine-phosphate adenylyltransferase -
  SK637_RS01610 (SK637_00316) sepM 307638..308675 (+) 1038 WP_033688116.1 SepM family pheromone-processing serine protease Regulator
  SK637_RS01615 (SK637_00317) - 308732..308962 (+) 231 WP_004260552.1 DUF1146 family protein -
  SK637_RS01620 (SK637_00318) murA 308986..310269 (+) 1284 WP_033688117.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SK637_RS01625 (SK637_00319) - 310262..310450 (+) 189 WP_004241151.1 DNA-directed RNA polymerase subunit beta -
  SK637_RS01630 (SK637_00320) endA 310489..311313 (+) 825 WP_033688119.1 DNA-entry nuclease EndA Machinery gene
  SK637_RS01635 (SK637_00321) - 311468..312799 (+) 1332 WP_033688120.1 hemolysin family protein -
  SK637_RS01655 (SK637_00324) amiA 313339..315318 (+) 1980 WP_033688121.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29968.57 Da        Isoelectric Point: 9.7812

>NTDB_id=284686 SK637_RS01630 WP_033688119.1 310489..311313(+) (endA) [Streptococcus mitis strain SK637]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQTTANSPKTKVSQKKQASEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQTEDSTGQNYYESMVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=284686 SK637_RS01630 WP_033688119.1 310489..311313(+) (endA) [Streptococcus mitis strain SK637]
ATGGATAAAAAAACAAGACAGACACTGATTGGACTGCTAGTGTTATTGCTTTTGTCTGCAGGGAGCTATTATATCAAGCA
GATGCAGACGACAGCAAATAGTCCTAAAACTAAGGTTAGTCAGAAAAAACAAGCTTCGGAAGCTCCTAGTCAAGAATTAG
CAGAAAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAAGGGAGTCTTGAGTGGAATGGCTCAGGTGCTTTTATTGTC
AATGGCAATAAAACAAACCTAGATGCCAAGGTTTCAAGTAAACCCTACGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACCGTGCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAAAATCGTGAAGAAACTGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTTGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAATATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCGCAAACTGAGGATTCGACAGGGCAAAACTACTATGAAAGCATGGTGCGTAAAGCCTTAGATC
AAAACAAGCGTGTCCGTTACCGTGTAACTCTTTATTATGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGATGGAGAATTGGAATTCAATGTCCTAGTTCCCAATGTTCAAAAGGGGCTTCAACTGGATTACCGAAC
TGGAGAAGTAACGGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

94.891

100

0.949
  endA Streptococcus pneumoniae D39

94.891

100

0.949
  endA Streptococcus pneumoniae R6

94.891

100

0.949
  endA Streptococcus pneumoniae TIGR4

94.891

100

0.949

Multiple sequence alignment