Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   C7M51_RS02385 Genome accession   NZ_CP028271
Coordinates   521507..522304 (-) Length   265 a.a.
NCBI ID   WP_160620170.1    Uniprot ID   -
Organism   Mixta intestinalis strain SRCM103226     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516507..527304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M51_RS02350 (C7M51_00467) - 516608..516955 (-) 348 WP_160620156.1 DUF1971 domain-containing protein -
  C7M51_RS02355 (C7M51_00468) - 516981..517319 (-) 339 WP_160620158.1 DUF1869 domain-containing protein -
  C7M51_RS02360 (C7M51_00469) - 517330..517662 (-) 333 WP_160620160.1 DUF1971 domain-containing protein -
  C7M51_RS02365 (C7M51_00470) - 518137..518496 (-) 360 WP_160620162.1 hypothetical protein -
  C7M51_RS02370 (C7M51_00471) - 518540..519433 (-) 894 WP_160620164.1 dihydrodipicolinate synthase family protein -
  C7M51_RS02375 (C7M51_00472) - 519680..520999 (+) 1320 WP_160620166.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  C7M51_RS02380 (C7M51_00473) gspS 521167..521529 (+) 363 WP_160620168.1 type II secretion system pilot lipoprotein GspS -
  C7M51_RS02385 (C7M51_00474) pilD 521507..522304 (-) 798 WP_160620170.1 A24 family peptidase Machinery gene
  C7M51_RS02390 (C7M51_00475) - 522304..522753 (-) 450 WP_160620172.1 general secretion pathway protein GspB -
  C7M51_RS02395 (C7M51_00476) - 522822..523472 (-) 651 WP_160620174.1 type II secretion system protein N -
  C7M51_RS02400 (C7M51_00477) gspM 523475..523918 (-) 444 WP_160620176.1 type II secretion system protein GspM -
  C7M51_RS02405 (C7M51_00478) gspL 523915..525048 (-) 1134 WP_160620178.1 type II secretion system protein GspL -
  C7M51_RS02410 (C7M51_00479) gspK 525048..525971 (-) 924 WP_160620180.1 type II secretion system minor pseudopilin GspK -
  C7M51_RS02415 (C7M51_00480) gspJ 525974..526564 (-) 591 WP_160620182.1 type II secretion system minor pseudopilin GspJ -
  C7M51_RS02420 (C7M51_00481) gspI 526561..526932 (-) 372 WP_160620184.1 type II secretion system minor pseudopilin GspI -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29845.97 Da        Isoelectric Point: 7.2388

>NTDB_id=283919 C7M51_RS02385 WP_160620170.1 521507..522304(-) (pilD) [Mixta intestinalis strain SRCM103226]
MIEIEWLAFMLLAGLLAGSVLNCVIWRLPVMMRRTSSDTQDDGFPERFNLFLPRSCCPHCLQTIPFYHNIPLLSWLLLRG
RCHHCHVSISLRYPLIELLTALCTGWIAWCWPPGWYALCLASGSWLLIALFAIDLEKMLLPDMLTLPLLWLGLLANLHGA
ITPLPDAVIGAVTGYLFLWIFYWGFLLITGKEGLGYGDFKLLAALGAWCGWQRLPILLITASLAGIGLFMLLRLTGKIRE
DGAFPFGPCLAIAGWWQMVMPLVMW

Nucleotide


Download         Length: 798 bp        

>NTDB_id=283919 C7M51_RS02385 WP_160620170.1 521507..522304(-) (pilD) [Mixta intestinalis strain SRCM103226]
ATGATCGAAATTGAATGGCTGGCGTTTATGTTATTAGCGGGCTTACTGGCTGGCAGCGTACTTAACTGTGTTATCTGGCG
CCTTCCTGTCATGATGAGGCGTACCAGCAGCGATACCCAGGATGACGGTTTTCCTGAACGCTTTAACCTGTTCCTGCCCC
GCTCATGCTGTCCTCACTGCCTGCAAACCATTCCCTTTTACCACAATATCCCGCTGCTAAGCTGGCTGCTGCTGCGCGGA
CGCTGCCATCATTGTCATGTTTCGATTAGCCTGCGTTATCCTCTTATTGAACTGCTTACCGCGCTTTGTACCGGCTGGAT
CGCCTGGTGCTGGCCGCCGGGATGGTATGCGCTTTGTCTCGCATCAGGTAGCTGGTTGCTGATCGCGCTTTTCGCTATCG
ATCTGGAAAAGATGCTGTTGCCCGATATGCTCACGCTGCCGTTGCTCTGGCTGGGGCTGTTGGCAAATCTGCACGGCGCA
ATTACACCATTACCCGATGCCGTAATCGGTGCCGTCACGGGCTATCTGTTTCTCTGGATTTTCTACTGGGGATTCCTGCT
AATCACCGGAAAAGAAGGCCTCGGCTACGGGGACTTTAAGCTGCTCGCCGCGCTGGGTGCCTGGTGCGGCTGGCAGCGGC
TACCTATCCTGTTAATAACGGCCTCGCTGGCGGGAATCGGCCTGTTCATGCTGTTACGCCTGACGGGAAAAATTAGAGAG
GACGGCGCTTTTCCCTTCGGCCCGTGCCTTGCTATCGCGGGCTGGTGGCAGATGGTTATGCCTTTGGTAATGTGGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(13-110)

Type IV leader peptidase family (Peptidase_A24)

(124-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.876

97.358

0.525
  pilD Vibrio campbellii strain DS40M4

49.057

100

0.491
  pilD Acinetobacter baumannii D1279779

47.451

96.226

0.457
  pilD Acinetobacter nosocomialis M2

47.451

96.226

0.457
  pilD Neisseria gonorrhoeae MS11

46.4

94.34

0.438

Multiple sequence alignment