Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   EFD52_RS10470 Genome accession   NZ_CP033606
Coordinates   2132885..2134855 (-) Length   656 a.a.
NCBI ID   WP_031297070.1    Uniprot ID   Q9CDL1
Organism   Lactococcus lactis strain IL6288     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2127885..2139855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD52_RS10445 (EFD52_10445) - 2128270..2129454 (-) 1185 WP_010906380.1 ABC transporter permease -
  EFD52_RS10455 (EFD52_10455) - 2130031..2130915 (-) 885 WP_010906381.1 XRE/MutR family transcriptional regulator -
  EFD52_RS10460 (EFD52_10460) ruvB 2131052..2132053 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EFD52_RS10465 (EFD52_10465) ruvA 2132179..2132772 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  EFD52_RS10470 (EFD52_10470) hexB 2132885..2134855 (-) 1971 WP_031297070.1 DNA mismatch repair endonuclease MutL Machinery gene
  EFD52_RS10475 (EFD52_10475) - 2134984..2135679 (-) 696 WP_010906384.1 hypothetical protein -
  EFD52_RS10480 (EFD52_10480) hexA 2135794..2138316 (-) 2523 WP_010906385.1 DNA mismatch repair protein MutS Machinery gene
  EFD52_RS10485 (EFD52_10485) - 2138510..2138857 (-) 348 WP_010906386.1 RicAFT regulatory complex protein RicA family protein -
  EFD52_RS10495 (EFD52_10495) - 2139226..2139528 (-) 303 WP_161908452.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 656 a.a.        Molecular weight: 73711.90 Da        Isoelectric Point: 5.1824

>NTDB_id=283810 EFD52_RS10470 WP_031297070.1 2132885..2134855(-) (hexB) [Lactococcus lactis strain IL6288]
MGKIIELNEALANQIAAGEVVERPASVVKELVENSIDAGSSKIIINVEEAGLRLIEVIDNGLGLEKEDVALALRRHATSK
IKDSADLFRIRTLGFRGEALPSIASVSQMTIETSTAEEESGTKLVAKGGNIETLEPLAKRVGTKISVANLFYNTPARLKY
IKSLQAELSHITDIINRLSLAHPEISFTLVNEGKEFLKTAGNGDLRQVIAAIYGIGTAKKMRQIKGSDLDFELTGYVSLP
ELTRANRNYITILINGRFIKNFLLNRAILEGYGNRLMVGRFPFAILSIKIDPTLADVNVHPTKQEVRLSKERELMALISK
AIDEALSEGVLIPEALENLQGRAKEKESLSVQTELPLQNNPLYYDNVRQDFYVREEATFKINKNQASNDSSEQTFDNFTE
NQLNTDAVSEKMTDRTVESTTEITDNSLENTVSNFEIDFDNEAKISQSSTFPQLEYLAQLHATYLLCQSKEGLYLVDQHA
AQERIKYEYWKDKIGEVSMEQQILLAPYLFTLAKNDFIVLAEKKDLLHEAGVFLEEYGENQFILREHPIWLKETEIEKSI
NEMIDIILSSKEFSLKKYRHDLAAMVACKSSIKANHPLDAESARALLAELATCKNPYSCAHGRPTIVHFSGDDIQKMFRR
IQETHRSKAASWKDFE

Nucleotide


Download         Length: 1971 bp        

>NTDB_id=283810 EFD52_RS10470 WP_031297070.1 2132885..2134855(-) (hexB) [Lactococcus lactis strain IL6288]
GTGGGAAAAATTATTGAACTAAATGAAGCGCTCGCCAATCAAATTGCTGCTGGAGAGGTGGTTGAGCGGCCTGCTAGTGT
TGTCAAAGAATTAGTCGAAAACTCAATTGATGCTGGAAGCAGTAAAATTATTATCAATGTTGAAGAAGCTGGTTTGCGGT
TAATTGAAGTCATTGATAATGGTTTGGGTTTGGAAAAAGAAGATGTGGCTTTGGCTTTACGTCGTCATGCAACAAGTAAA
ATCAAGGATTCGGCAGATTTGTTTCGGATTAGAACACTTGGTTTTCGGGGGGAGGCTCTGCCATCAATCGCTTCTGTCAG
CCAGATGACCATTGAAACAAGTACGGCTGAGGAAGAATCAGGAACAAAACTAGTTGCTAAAGGTGGAAATATCGAAACTT
TAGAACCACTTGCTAAGCGAGTGGGGACGAAAATTTCTGTTGCTAATCTTTTTTATAATACACCAGCAAGACTCAAGTAT
ATCAAGTCCTTGCAGGCTGAACTTTCTCATATCACAGACATCATCAACCGATTGAGTCTGGCTCATCCAGAGATTTCCTT
TACTTTAGTTAATGAGGGTAAAGAATTTTTGAAAACGGCTGGAAATGGTGATTTACGCCAGGTTATTGCTGCAATTTATG
GCATAGGGACAGCGAAAAAAATGCGTCAAATCAAGGGCTCAGACTTAGATTTTGAACTAACAGGTTATGTCAGCTTACCA
GAGCTGACAAGGGCTAATCGTAATTATATTACGATTTTAATAAATGGTCGATTTATCAAAAACTTTTTATTGAATCGGGC
AATTTTAGAAGGATATGGCAATCGATTGATGGTGGGACGGTTCCCTTTTGCTATTTTATCGATTAAAATTGACCCAACAC
TTGCTGATGTTAATGTTCATCCGACAAAACAAGAGGTGCGTTTGTCTAAAGAGCGTGAATTGATGGCTTTAATTTCTAAA
GCAATTGATGAAGCTTTATCAGAAGGTGTTTTGATTCCAGAAGCTTTGGAAAATTTGCAAGGAAGAGCTAAAGAAAAGGA
GAGTCTTTCTGTTCAAACGGAACTTCCTTTGCAAAATAATCCTTTATATTATGATAATGTTCGCCAAGATTTTTATGTCA
GAGAAGAGGCAACTTTTAAAATCAATAAAAATCAAGCTTCAAATGATTCGTCTGAACAAACTTTTGATAATTTTACAGAA
AATCAACTTAATACAGATGCTGTCAGTGAAAAAATGACTGACAGAACTGTTGAAAGTACTACTGAAATTACTGACAACAG
TCTAGAAAATACTGTCAGCAACTTTGAAATTGATTTTGATAATGAAGCAAAGATAAGTCAGTCATCAACTTTTCCGCAGC
TTGAATACTTGGCACAATTGCATGCGACTTATTTACTTTGTCAGTCCAAAGAAGGACTTTATTTGGTTGACCAACATGCG
GCTCAAGAGCGAATAAAATATGAGTATTGGAAAGATAAAATTGGTGAGGTCAGTATGGAGCAACAAATTTTACTTGCACC
ATATTTGTTCACTTTAGCCAAAAATGATTTTATTGTTTTAGCCGAGAAGAAAGATTTGTTGCATGAAGCCGGTGTTTTCT
TAGAAGAATACGGAGAAAATCAATTCATTTTAAGAGAACATCCAATCTGGTTAAAAGAAACCGAGATAGAGAAATCAATT
AATGAAATGATTGATATTATTCTCTCATCAAAAGAATTTTCACTCAAAAAATATCGTCATGATTTAGCTGCAATGGTTGC
TTGTAAAAGTTCAATTAAAGCTAATCATCCCCTTGACGCCGAGTCAGCTAGAGCTTTACTCGCAGAATTAGCAACTTGTA
AAAATCCTTATAGTTGTGCGCATGGACGGCCAACGATTGTTCATTTTTCAGGGGATGACATTCAAAAAATGTTCCGGAGA
ATCCAAGAAACGCATCGTTCAAAAGCAGCTTCGTGGAAAGATTTTGAGTAA

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-123)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-326)

MutL C terminal dimerisation domain (MutL_C)

(455-598)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

57.143

99.238

0.567