Detailed information NTG0028361

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: TMEL_RS08005
• Genome accession: NC_009616
• Coordinates: 1543150..1544037 (+)
• Length: 295 a.a.
• NCBI ID: WP_012057759.1
• Uniprot ID: A6LN99
• Organism: Thermosipho melanesiensis BI429
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1538150..1549037

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TMEL_RS07970 (Tmel_1548)	-	1538403..1539305 (+)	903	WP_012057752.1	PEGA domain-containing protein	-
TMEL_RS07975 (Tmel_1549)	prfB	1539324..1540433 (+)	1110	WP_012057753.1	peptide chain release factor 2	-
TMEL_RS07980 (Tmel_1550)	minD	1540444..1541247 (+)	804	WP_012057754.1	septum site-determining protein MinD	-
TMEL_RS07985 (Tmel_1551)	-	1541253..1541519 (+)	267	WP_012057755.1	trigger factor protein	-
TMEL_RS07990 (Tmel_1552)	-	1541465..1542133 (+)	669	WP_041426104.1	hypothetical protein	-
TMEL_RS07995 (Tmel_1553)	-	1542145..1542369 (+)	225	WP_012057757.1	hypothetical protein	-
TMEL_RS08000 (Tmel_1554)	-	1542366..1543148 (+)	783	WP_012057758.1	class I SAM-dependent methyltransferase	-
TMEL_RS08005 (Tmel_1555)	pilA	1543150..1544037 (+)	888	WP_012057759.1	signal recognition particle-docking protein FtsY	Machinery gene
TMEL_RS08010 (Tmel_1556)	-	1544039..1544707 (+)	669	WP_012057760.1	DUF2225 domain-containing protein	-
TMEL_RS08015 (Tmel_1557)	-	1544686..1546266 (+)	1581	WP_041426105.1	3D domain-containing protein	-
TMEL_RS08020 (Tmel_1558)	-	1546269..1546715 (+)	447	WP_012057762.1	RrF2 family transcriptional regulator	-
TMEL_RS08025 (Tmel_1559)	mnmA	1546712..1547788 (+)	1077	WP_041426106.1	tRNA 2-thiouridine(34) synthase MnmA	-
TMEL_RS08030 (Tmel_1560)	hpt	1547766..1548278 (+)	513	WP_012057764.1	hypoxanthine phosphoribosyltransferase	-

Sequence

Protein

• Length: 295 a.a.
• Molecular weight: 32212.17 Da
• Isoelectric Point: 5.3297

>NTDB_id=28361 TMEL_RS08005 WP_012057759.1 1543150..1544037(+) (pilA) [Thermosipho melanesiensis BI429]
MGFFEKFKKGLQKTRETFFGKIKEVLSGKKLDEEVKEEIEELLILSDVGVEATEYIIKKIEESSGEDAYETLKKVLVEML
SDNTELNLFEKPTVINMVGVNGSGKTTTAGKLASYFSKNGKSVVLAACDTFRAAAIDQLKVWGERTNSTVIAHQEGADSA
AVAFDAVNHAISKNKDIVILDTAGRLHTKKNLMEELRKINRVIKKKLESAPHEILLVIDAVTGQNGLQQAKIFKEFVNIT
GIVLTKLDGTAKGGIAIAIVKELGIPIKFVGLGEGVDDLKPFDAEDFVNALLEGE

Nucleotide

• Length: 888 bp

>NTDB_id=28361 TMEL_RS08005 WP_012057759.1 1543150..1544037(+) (pilA) [Thermosipho melanesiensis BI429]
ATGGGTTTTTTTGAGAAATTTAAAAAAGGCCTTCAAAAAACAAGAGAAACCTTTTTTGGAAAGATAAAGGAGGTGTTGAG
TGGGAAAAAATTAGATGAAGAGGTAAAAGAAGAAATTGAGGAGCTTTTGATATTATCAGATGTTGGAGTTGAAGCAACAG
AGTATATTATTAAGAAAATTGAGGAAAGCAGTGGGGAAGATGCTTATGAAACTTTAAAGAAAGTATTAGTTGAGATGTTA
AGTGATAATACTGAATTAAATTTATTTGAAAAGCCTACTGTAATTAATATGGTTGGAGTTAATGGTTCGGGGAAGACTAC
TACTGCTGGAAAGTTGGCATCATATTTTTCAAAAAATGGGAAATCTGTTGTTTTAGCTGCGTGCGATACTTTTAGAGCAG
CCGCAATTGATCAATTAAAGGTTTGGGGTGAGAGGACAAATTCAACTGTAATTGCTCACCAAGAAGGAGCAGATTCAGCG
GCAGTTGCTTTTGATGCTGTAAATCATGCTATCTCGAAAAATAAAGATATTGTAATTCTTGATACCGCGGGTAGATTGCA
TACTAAAAAGAATCTAATGGAAGAATTAAGAAAGATAAATAGGGTGATAAAGAAAAAGTTAGAAAGTGCACCCCATGAAA
TATTATTGGTGATTGATGCTGTTACAGGGCAGAATGGATTACAACAGGCAAAAATATTTAAAGAATTTGTGAATATAACT
GGTATTGTGTTGACAAAGTTGGATGGTACTGCAAAGGGAGGAATTGCAATTGCGATAGTAAAAGAATTGGGAATTCCTAT
TAAGTTTGTTGGTCTAGGGGAAGGAGTTGATGATTTAAAACCGTTTGACGCAGAAGATTTTGTTAATGCATTGTTAGAAG
GTGAATAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (92-292)

• SRP54-type protein, helical bundle domain (SRP54_N) (19-75)

3D structure

• AlphaFold DB: A6LN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	46.865	100	0.481