Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   C7M30_RS18865 Genome accession   NZ_CP028218
Coordinates   3657001..3657768 (+) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain SRCM102756     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3652001..3662768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M30_RS18835 (C7M30_03736) pgpH 3652297..3654432 (+) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -
  C7M30_RS18840 (C7M30_03737) ybeY 3654433..3654906 (+) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  C7M30_RS18845 (C7M30_03738) unpK 3654887..3655258 (+) 372 WP_015384119.1 diacylglycerol kinase family protein -
  C7M30_RS18850 (C7M30_03739) - 3655377..3655787 (+) 411 WP_003230049.1 cytidine deaminase -
  C7M30_RS18855 (C7M30_03740) era 3655768..3656673 (+) 906 WP_003230051.1 GTPase Era -
  C7M30_RS18860 (C7M30_03741) yqzL 3656821..3656964 (+) 144 WP_003230053.1 YqzL family protein -
  C7M30_RS18865 (C7M30_03742) recO 3657001..3657768 (+) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  C7M30_RS18870 (C7M30_03743) glyQ 3658066..3658953 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  C7M30_RS18875 (C7M30_03744) glyS 3658946..3660985 (+) 2040 WP_144500706.1 glycine--tRNA ligase subunit beta -
  C7M30_RS18880 (C7M30_03745) ccpN 3661118..3661756 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  C7M30_RS18885 (C7M30_03746) ppsR 3661782..3662594 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=283457 C7M30_RS18865 WP_014477369.1 3657001..3657768(+) (recO) [Bacillus subtilis strain SRCM102756]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=283457 C7M30_RS18865 WP_014477369.1 3657001..3657768(+) (recO) [Bacillus subtilis strain SRCM102756]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCAATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGTAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTACTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment