Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   D8668_RS05460 Genome accession   NZ_CP033439
Coordinates   1173184..1173723 (+) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SP4528     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1168184..1178723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8668_RS05435 (D8668_05435) yacG 1168351..1168551 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  D8668_RS05440 (D8668_05440) coaE 1168548..1169159 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  D8668_RS05445 (D8668_05445) pilD 1169156..1170028 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  D8668_RS05450 (D8668_05450) pilC 1170031..1171248 (-) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  D8668_RS05455 (D8668_05455) pilB 1171252..1172955 (-) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D8668_RS05460 (D8668_05460) pilE 1173184..1173723 (+) 540 WP_033938465.1 pilin Machinery gene
  D8668_RS05465 (D8668_05465) - 1173928..1174485 (+) 558 WP_224754157.1 hypothetical protein -
  D8668_RS05475 (D8668_05475) nadC 1174649..1175497 (-) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D8668_RS05480 (D8668_05480) - 1175783..1178038 (+) 2256 WP_033990664.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=283416 D8668_RS05460 WP_033938465.1 1173184..1173723(+) (pilE) [Pseudomonas aeruginosa strain SP4528]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=283416 D8668_RS05460 WP_033938465.1 1173184..1173723(+) (pilE) [Pseudomonas aeruginosa strain SP4528]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363

Multiple sequence alignment