Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   EB670_RS09740 Genome accession   NZ_CP033378
Coordinates   1974498..1976330 (+) Length   610 a.a.
NCBI ID   WP_001283421.1    Uniprot ID   P0A8G1
Organism   Escherichia coli strain L73     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1969498..1981330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB670_RS09705 (EB670_09705) dcyD 1969725..1970711 (+) 987 WP_001128238.1 D-cysteine desulfhydrase -
  EB670_RS09710 (EB670_09710) tcyL 1970726..1971394 (+) 669 WP_001158220.1 cystine ABC transporter permease -
  EB670_RS09715 (EB670_09715) tcyN 1971391..1972143 (+) 753 WP_001272994.1 L-cystine ABC transporter ATP-binding protein TcyN -
  EB670_RS09720 (EB670_09720) sdiA 1972373..1973095 (+) 723 WP_001154276.1 transcriptional regulator SdiA -
  EB670_RS09725 (EB670_09725) yecF 1973162..1973386 (-) 225 WP_000106474.1 DUF2594 family protein YecF -
  EB670_RS09730 (EB670_09730) yecU 1973373..1973549 (-) 177 WP_000590347.1 protein YecU -
  EB670_RS25565 - 1973632..1973703 (-) 72 Protein_1888 transcriptional regulator -
  EB670_RS09735 (EB670_09735) letA 1973845..1974501 (+) 657 WP_000611335.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  EB670_RS09740 (EB670_09740) uvrC 1974498..1976330 (+) 1833 WP_001283421.1 excinuclease ABC subunit UvrC Machinery gene
  EB670_RS09745 (EB670_09745) pgsA 1976387..1976935 (+) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  EB670_RS09770 (EB670_09770) - 1977631..1978371 (-) 741 WP_000413228.1 hypothetical protein -
  EB670_RS09775 (EB670_09775) - 1978452..1979651 (+) 1200 WP_001172374.1 site-specific integrase -
  EB670_RS09785 (EB670_09785) - 1980020..1980391 (+) 372 WP_000673980.1 hypothetical protein -
  EB670_RS09790 (EB670_09790) - 1980417..1980950 (+) 534 WP_000045625.1 hypothetical protein -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68188.03 Da        Isoelectric Point: 9.3685

>NTDB_id=283271 EB670_RS09740 WP_001283421.1 1974498..1976330(+) (uvrC) [Escherichia coli strain L73]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=283271 EB670_RS09740 WP_001283421.1 1974498..1976330(+) (uvrC) [Escherichia coli strain L73]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCACACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCTTATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCGAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATATTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGT
AATCGCTCGCGTCCGTGTCTGCAATATCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTGATTAGCCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCGGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAGTTTGTTTCCAATACCGGCGACGACCTCGACGTTATTGGTGTGGCGTTCGATGCGGGCATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTAGAAACCTTCGTAGGCCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGCGGCGATAGGGCGCGTTATCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGACTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGCGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACAGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TTGACGACAGTAAGATCCCGGATGTGATCCTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCACTGCTACTTGGCGTTGCCAAAGGAGCAGATCGTAAGGCTGGGCTGGA
AACACTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACACGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGCTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGTCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A8G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374

Multiple sequence alignment