Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C7M28_RS03810 Genome accession   NZ_CP028215
Coordinates   704311..704721 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM102750     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 699311..709721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M28_RS03780 - 699398..699538 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  C7M28_RS03785 (C7M28_00752) yqeF 699844..700575 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  C7M28_RS03790 (C7M28_00753) cwlH 700827..701579 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  C7M28_RS03795 (C7M28_00754) yqeD 701766..702392 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  C7M28_RS03800 (C7M28_00755) gnd 702411..703304 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  C7M28_RS03805 (C7M28_00756) yqeB 703556..704278 (+) 723 WP_046381199.1 hypothetical protein -
  C7M28_RS03810 (C7M28_00757) nucA/comI 704311..704721 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  C7M28_RS03815 (C7M28_00758) sigK 704917..705645 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  C7M28_RS03820 (C7M28_00759) - 706205..706885 (-) 681 WP_046381200.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
  C7M28_RS03825 (C7M28_00760) - 707016..708359 (+) 1344 WP_046381201.1 MATE family efflux transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=283242 C7M28_RS03810 WP_009967785.1 704311..704721(-) (nucA/comI) [Bacillus subtilis strain SRCM102750]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=283242 C7M28_RS03810 WP_009967785.1 704311..704721(-) (nucA/comI) [Bacillus subtilis strain SRCM102750]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment