Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DA787_RS12975 Genome accession   NZ_CP033205
Coordinates   2511271..2511681 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain MBI 600     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2506271..2516681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA787_RS12945 (DA787_12930) - 2506358..2506498 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  DA787_RS12950 (DA787_12935) yqeF 2506804..2507535 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  DA787_RS12955 (DA787_12940) cwlH 2507787..2508539 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  DA787_RS12960 (DA787_12945) yqeD 2508726..2509352 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  DA787_RS12965 (DA787_12950) gnd 2509371..2510264 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  DA787_RS12970 (DA787_12955) yqeB 2510516..2511238 (+) 723 WP_010886572.1 hypothetical protein -
  DA787_RS12975 (DA787_12960) nucA/comI 2511271..2511681 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  DA787_RS12980 (DA787_12965) - 2511877..2512224 (+) 348 Protein_2486 sigma-70 family RNA polymerase sigma factor -
  DA787_RS12985 (DA787_12970) spoIVCA 2512255..2513715 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  DA787_RS21440 (DA787_12975) - 2513673..2513851 (-) 179 Protein_2488 hypothetical protein -
  DA787_RS12995 (DA787_12980) arsC 2514206..2514625 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  DA787_RS13000 (DA787_12985) acr3 2514637..2515677 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  DA787_RS13005 (DA787_12990) arsK 2515700..2516140 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  DA787_RS13010 (DA787_12995) arsR 2516201..2516518 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=282598 DA787_RS12975 WP_009967785.1 2511271..2511681(-) (nucA/comI) [Bacillus subtilis strain MBI 600]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=282598 DA787_RS12975 WP_009967785.1 2511271..2511681(-) (nucA/comI) [Bacillus subtilis strain MBI 600]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529