Detailed information NTG0282548

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M19_RS03885
• Genome accession: NZ_CP028205
• Coordinates: 859314..859928 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102741
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 854314..864928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M19_RS03855 (C7M19_00787)	rpsT	854617..854883 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C7M19_RS03860 (C7M19_00788)	holA	854900..855941 (-)	1042	Protein_781	DNA polymerase III subunit delta	-
C7M19_RS03865	-	855981..856132 (-)	152	Protein_782	hypothetical protein	-
C7M19_RS03870	-	856173..856307 (+)	135	WP_003152870.1	YqzM family protein	-
C7M19_RS03875 (C7M19_00789)	comEC	856326..858677 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M19_RS03880 (C7M19_00790)	-	858678..859247 (-)	570	WP_094031848.1	ComE operon protein 2	-
C7M19_RS03885 (C7M19_00791)	comEA	859314..859928 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M19_RS03890 (C7M19_00792)	comER	859987..860808 (+)	822	WP_012118027.1	late competence protein ComER	-
C7M19_RS03895 (C7M19_00793)	-	860877..861614 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
C7M19_RS03900 (C7M19_00794)	rsfS	861611..861967 (-)	357	WP_007408260.1	ribosome silencing factor	-
C7M19_RS03905 (C7M19_00795)	yqeK	861985..862545 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M19_RS03910 (C7M19_00796)	-	862535..863104 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C7M19_RS03915 (C7M19_00797)	yhbY	863115..863405 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
C7M19_RS03920 (C7M19_00798)	aroE	863399..864241 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=282548 C7M19_RS03885 WP_043021637.1 859314..859928(-) (comEA) [Bacillus velezensis strain SRCM102741]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=282548 C7M19_RS03885 WP_043021637.1 859314..859928(-) (comEA) [Bacillus velezensis strain SRCM102741]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363