Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C7M16_RS20025 Genome accession   NZ_CP028201
Coordinates   3945345..3946721 (+) Length   458 a.a.
NCBI ID   WP_014475593.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM102753     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3940345..3951721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M16_RS20005 (C7M16_03944) ctsR 3940698..3941162 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  C7M16_RS20010 mcsA 3941176..3941733 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  C7M16_RS20015 (C7M16_03946) mcsB 3941733..3942824 (+) 1092 WP_003235007.1 protein arginine kinase -
  C7M16_RS20020 (C7M16_03947) clpC 3942821..3945253 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C7M16_RS20025 (C7M16_03948) radA 3945345..3946721 (+) 1377 WP_014475593.1 DNA repair protein RadA Machinery gene
  C7M16_RS20030 (C7M16_03949) disA 3946725..3947807 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  C7M16_RS20035 (C7M16_03950) yacL 3947923..3949023 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  C7M16_RS20040 (C7M16_03951) ispD 3949038..3949736 (+) 699 WP_106074229.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C7M16_RS20045 (C7M16_03952) ispF 3949729..3950205 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49466.99 Da        Isoelectric Point: 8.2399

>NTDB_id=282376 C7M16_RS20025 WP_014475593.1 3945345..3946721(+) (radA) [Bacillus subtilis strain SRCM102753]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=282376 C7M16_RS20025 WP_014475593.1 3945345..3946721(+) (radA) [Bacillus subtilis strain SRCM102753]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCAGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTATCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGAGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ATTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.782

100

0.998
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

62.914

98.908

0.622

Multiple sequence alignment