Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   C9J79_RS08735 Genome accession   NZ_CP028185
Coordinates   1674775..1675734 (+) Length   319 a.a.
NCBI ID   WP_070309048.1    Uniprot ID   -
Organism   Campylobacter jejuni strain CFSAN054107     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1658975..1679777 1674775..1675734 within 0


Gene organization within MGE regions


Location: 1658975..1679777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9J79_RS08665 (C9J79_08660) pglI 1658975..1659904 (-) 930 WP_032583777.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  C9J79_RS08670 (C9J79_08665) pglH 1659897..1660976 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  C9J79_RS08675 (C9J79_08670) pglK 1660973..1662667 (-) 1695 WP_070318342.1 ABC-type lipopolysaccharide transporter PglK -
  C9J79_RS08680 (C9J79_08675) galE 1662661..1663647 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  C9J79_RS08685 (C9J79_08680) - 1663706..1664500 (-) 795 WP_070318343.1 3'-5' exonuclease -
  C9J79_RS08690 (C9J79_08685) waaC 1664567..1665595 (+) 1029 WP_107131199.1 lipopolysaccharide heptosyltransferase I -
  C9J79_RS08695 (C9J79_08690) htrB 1665585..1666472 (+) 888 WP_002871713.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  C9J79_RS08700 (C9J79_08695) - 1666469..1668019 (+) 1551 WP_032591562.1 glycosyltransferase -
  C9J79_RS08705 (C9J79_08700) - 1668023..1669201 (+) 1179 WP_032591565.1 glycosyltransferase family 9 protein -
  C9J79_RS08710 (C9J79_08705) - 1669198..1670337 (+) 1140 WP_032591568.1 glycosyltransferase family 4 protein -
  C9J79_RS08715 (C9J79_08710) - 1670334..1671533 (-) 1200 WP_032591570.1 glycosyltransferase family 8 protein -
  C9J79_RS08720 (C9J79_08715) - 1671526..1672677 (-) 1152 WP_032591573.1 glycosyltransferase family 8 protein -
  C9J79_RS08725 (C9J79_08720) - 1672690..1673838 (-) 1149 WP_079344032.1 FkbM family methyltransferase -
  C9J79_RS08730 (C9J79_08725) waaV 1673901..1674716 (-) 816 WP_060806422.1 glycosyltransferase family 2 protein -
  C9J79_RS08735 (C9J79_08730) waaF 1674775..1675734 (+) 960 WP_070309048.1 lipopolysaccharide heptosyltransferase II Regulator
  C9J79_RS08740 (C9J79_08735) gmhA 1675715..1676275 (-) 561 WP_020247045.1 D-sedoheptulose 7-phosphate isomerase -
  C9J79_RS08745 (C9J79_08740) rfaE1 1676272..1677657 (-) 1386 WP_107131200.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  C9J79_RS08750 (C9J79_08745) rfaD 1677650..1678603 (-) 954 WP_002860242.1 ADP-glyceromanno-heptose 6-epimerase -
  C9J79_RS08755 (C9J79_08750) gmhB 1678604..1679165 (-) 562 Protein_1700 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  C9J79_RS08760 (C9J79_08755) - 1679246..1679548 (+) 303 WP_002852762.1 cytochrome c -
  C9J79_RS08765 (C9J79_08760) ccoS 1679571..1679777 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36789.44 Da        Isoelectric Point: 10.4034

>NTDB_id=282119 C9J79_RS08735 WP_070309048.1 1674775..1675734(+) (waaF) [Campylobacter jejuni strain CFSAN054107]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQVLSLRKKLGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSLLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCDEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GSMHISAVYKVKTVAIFGPTKFTQTSPWQNENARWVHLNLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNPHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=282119 C9J79_RS08735 WP_070309048.1 1674775..1675734(+) (waaF) [Campylobacter jejuni strain CFSAN054107]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGTCCTATCTTTACGCAAAAAACTTGGCAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCTTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGTCAAAGTCATGAAATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTCTGCGATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAACCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGTGATCTTTTCATCACAAATGACAGT
GGCTCTATGCACATAAGTGCGGTTTACAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAATGCAAGATGGGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTCAAAGGGTTATAGAAGAAGCTAGAAATTTACTTAAAAACCCTCATCTATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

91.85

100

0.919

Multiple sequence alignment