Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LL14B4_RS11580 Genome accession   NZ_CP028160
Coordinates   2340329..2341690 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain 14B4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2335329..2346690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL14B4_RS11545 (LL14B4_11535) - 2335875..2336369 (+) 495 WP_012898594.1 GNAT family N-acetyltransferase -
  LL14B4_RS11550 (LL14B4_11540) - 2336462..2336785 (-) 324 WP_003129893.1 DUF1048 domain-containing protein -
  LL14B4_RS11555 (LL14B4_11545) - 2336772..2337119 (-) 348 WP_012898595.1 DUF1048 domain-containing protein -
  LL14B4_RS11560 (LL14B4_11550) - 2337106..2337468 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  LL14B4_RS11565 (LL14B4_11555) - 2337589..2338218 (-) 630 WP_003129888.1 hypothetical protein -
  LL14B4_RS11570 (LL14B4_11560) - 2338283..2339368 (-) 1086 WP_033900742.1 PIN/TRAM domain-containing protein -
  LL14B4_RS11575 (LL14B4_11565) - 2339599..2340285 (+) 687 WP_109991334.1 YoaK family protein -
  LL14B4_RS11580 (LL14B4_11570) radA 2340329..2341690 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  LL14B4_RS11585 (LL14B4_11575) - 2341852..2342079 (-) 228 WP_010906294.1 heavy-metal-associated domain-containing protein -
  LL14B4_RS11600 (LL14B4_11590) rplQ 2342894..2343274 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  LL14B4_RS11605 (LL14B4_11595) - 2343293..2344231 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  LL14B4_RS11610 (LL14B4_11600) rpsK 2344279..2344662 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  LL14B4_RS11615 (LL14B4_11605) rpsM 2344683..2345048 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  LL14B4_RS11620 (LL14B4_11610) rpmJ 2345066..2345182 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  LL14B4_RS11625 (LL14B4_11615) infA 2345236..2345454 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  LL14B4_RS11630 (LL14B4_11620) - 2345751..2346398 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=281824 LL14B4_RS11580 WP_003130563.1 2340329..2341690(-) (radA) [Lactococcus lactis subsp. lactis strain 14B4]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=281824 LL14B4_RS11580 WP_003130563.1 2340329..2341690(-) (radA) [Lactococcus lactis subsp. lactis strain 14B4]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGTATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGATCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TAACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAAAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment