Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TPET_RS01750 Genome accession   NC_009486
Coordinates   333296..334180 (+) Length   294 a.a.
NCBI ID   WP_011943012.1    Uniprot ID   A5IJK4
Organism   Thermotoga petrophila RKU-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328296..339180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TPET_RS01735 (Tpet_0345) lnt 329099..330562 (-) 1464 WP_011943009.1 apolipoprotein N-acyltransferase -
  TPET_RS01740 (Tpet_0346) - 330555..331706 (-) 1152 WP_011943010.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  TPET_RS01745 (Tpet_0347) - 331803..333182 (-) 1380 WP_011943011.1 DegQ family serine endoprotease -
  TPET_RS01750 (Tpet_0348) pilA 333296..334180 (+) 885 WP_011943012.1 signal recognition particle-docking protein FtsY Machinery gene
  TPET_RS01755 (Tpet_0349) - 334177..334833 (+) 657 WP_011943013.1 DUF2225 domain-containing protein -
  TPET_RS01760 (Tpet_0350) - 334809..336473 (+) 1665 WP_048810849.1 peptidoglycan DD-metalloendopeptidase family protein -
  TPET_RS01765 (Tpet_0351) - 336475..336888 (+) 414 WP_011943015.1 Rrf2 family transcriptional regulator -
  TPET_RS01770 (Tpet_0352) - 336907..337101 (-) 195 WP_011943016.1 hypothetical protein -
  TPET_RS01775 (Tpet_0353) sfsA 337105..337773 (-) 669 WP_011943017.1 DNA/RNA nuclease SfsA -
  TPET_RS01780 (Tpet_0354) - 337770..338249 (-) 480 WP_011943018.1 flavin reductase family protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32016.22 Da        Isoelectric Point: 5.5192

>NTDB_id=28153 TPET_RS01750 WP_011943012.1 333296..334180(+) (pilA) [Thermotoga petrophila RKU-1]
MGLFDFLKKGLQKTKETFFGRVVKLLKGKKLDDETREELEELLIQADVGVETTEYILERLKEKDGDALESLKEIILEILN
FDTKLNVPPEPPFVITVVGVNGTGKTTSCGKLAKMFVDEGKSVVLAAADTFRAAAIEQLKIWGERVGATVISHSEGADPA
AVAFDAVAHALARNKDVVIIDTAGRLHTKKNLMEELRKVHRVVKKKIPDAPHETLLVIDATTGQNGLVQAKIFKEAVDVT
GIILTKLDGTAKGGITLAIARELGIPIKFIGVGEKAEDLRPFDPEAFVEVLISE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=28153 TPET_RS01750 WP_011943012.1 333296..334180(+) (pilA) [Thermotoga petrophila RKU-1]
ATGGGACTCTTCGATTTTTTGAAAAAGGGACTCCAGAAGACGAAAGAAACGTTTTTTGGACGAGTGGTGAAGCTCCTCAA
AGGAAAAAAGCTGGACGATGAAACCCGCGAGGAGCTGGAAGAACTTCTCATTCAAGCGGACGTGGGAGTCGAGACAACGG
AATACATTCTGGAGAGATTGAAAGAGAAGGACGGAGACGCTCTCGAGTCTCTCAAGGAAATCATTCTGGAAATTCTGAAC
TTCGATACGAAGCTGAACGTTCCTCCTGAACCTCCGTTTGTGATCACGGTTGTTGGTGTCAACGGAACGGGAAAGACCAC
CTCTTGCGGAAAGCTCGCAAAGATGTTCGTGGATGAAGGAAAGAGTGTGGTGCTCGCTGCGGCGGATACTTTCAGAGCGG
CCGCCATAGAACAGCTGAAGATATGGGGAGAACGTGTCGGTGCCACGGTGATATCCCACTCGGAAGGAGCAGATCCGGCC
GCCGTTGCCTTTGACGCGGTGGCTCACGCTCTCGCCAGAAACAAAGATGTGGTCATAATAGACACCGCCGGAAGACTTCA
CACGAAGAAGAATCTCATGGAGGAATTGCGGAAGGTTCACAGAGTTGTCAAAAAGAAGATCCCCGACGCTCCGCACGAAA
CGCTCCTCGTGATAGACGCCACCACCGGCCAAAACGGACTTGTTCAGGCAAAAATATTCAAAGAAGCGGTGGATGTCACC
GGTATAATTCTCACAAAGCTCGACGGAACCGCGAAAGGCGGAATAACTCTGGCCATTGCCCGGGAACTGGGAATACCCAT
AAAGTTCATCGGTGTCGGAGAAAAGGCAGAAGACCTGAGGCCTTTCGATCCTGAAGCCTTTGTGGAGGTATTGATTTCAG
AATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-292)

SRP54-type protein, helical bundle domain (SRP54_N)

(22-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A5IJK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.669

100

0.51

Multiple sequence alignment