Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   D9V56_RS00520 Genome accession   NZ_CP032986
Coordinates   102964..103632 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain BE2-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 97964..108632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9V56_RS00490 yicC 97984..98847 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  D9V56_RS00495 rph 98974..99690 (+) 717 WP_001247093.1 ribonuclease PH -
  D9V56_RS00500 pyrE 99755..100396 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  D9V56_RS00505 slmA 100433..101029 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  D9V56_RS00510 dut 101136..101594 (-) 459 WP_000976070.1 dUTP diphosphatase -
  D9V56_RS00515 coaBC 101572..102792 (-) 1221 WP_000050117.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  D9V56_RS00520 radC 102964..103632 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  D9V56_RS00525 rpmB 103849..104085 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  D9V56_RS00530 rpmG 104106..104273 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  D9V56_RS00535 mutM 104371..105180 (+) 810 WP_121689804.1 DNA-formamidopyrimidine glycosylase -
  D9V56_RS00540 coaD 105219..105698 (-) 480 WP_032231556.1 pantetheine-phosphate adenylyltransferase -
  D9V56_RS00545 waaA 105706..106983 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  D9V56_RS00550 rfaQ 107396..108454 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=281247 D9V56_RS00520 WP_001298959.1 102964..103632(+) (radC) [Escherichia coli strain BE2-5]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=281247 D9V56_RS00520 WP_001298959.1 102964..103632(+) (radC) [Escherichia coli strain BE2-5]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment