Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VC0395_RS16400 Genome accession   NC_009457
Coordinates   2357878..2358462 (-) Length   194 a.a.
NCBI ID   WP_000902745.1    Uniprot ID   A0A0H6BVQ2
Organism   Vibrio cholerae O395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2352878..2363462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VC0395_RS16375 (VC0395_A2205) aroB 2353222..2354307 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  VC0395_RS16380 (VC0395_A2206) aroK 2354335..2354859 (-) 525 WP_000818616.1 shikimate kinase AroK -
  VC0395_RS16385 (VC0395_A2207) pilQ 2355046..2356761 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  VC0395_RS16390 (VC0395_A2208) pilP 2356787..2357305 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VC0395_RS16395 (VC0395_A2209) pilO 2357295..2357885 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VC0395_RS16400 (VC0395_A2210) pilN 2357878..2358462 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VC0395_RS16405 (VC0395_A2211) pilM 2358452..2359468 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  VC0395_RS16410 (VC0395_A2212) - 2359611..2362088 (+) 2478 WP_000668744.1 penicillin-binding protein 1A -
  VC0395_RS16415 (VC0395_A2213) oxyR 2362162..2363055 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 21964.42 Da        Isoelectric Point: 9.9503

>NTDB_id=28095 VC0395_RS16400 WP_000902745.1 2357878..2358462(-) (pilN) [Vibrio cholerae O395]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQAGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=28095 VC0395_RS16400 WP_000902745.1 2357878..2358462(-) (pilN) [Vibrio cholerae O395]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCAGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAACAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGCGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H6BVQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

100

100

1
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552

Multiple sequence alignment