Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C7M59_RS18560 Genome accession   NZ_CP027780
Coordinates   3904218..3905576 (-) Length   452 a.a.
NCBI ID   WP_061326967.1    Uniprot ID   -
Organism   Clostridium botulinum strain Mfbjulcb3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3899218..3910576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M59_RS18540 (C7M59_18520) ispD 3900516..3901205 (-) 690 WP_061326965.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C7M59_RS18545 (C7M59_18525) - 3901207..3902307 (-) 1101 WP_003482883.1 PIN/TRAM domain-containing protein -
  C7M59_RS18550 (C7M59_18530) - 3902708..3903106 (+) 399 WP_030037311.1 hypothetical protein -
  C7M59_RS18555 (C7M59_18535) disA 3903138..3904199 (-) 1062 WP_030037313.1 DNA integrity scanning diadenylate cyclase DisA -
  C7M59_RS18560 (C7M59_18540) radA 3904218..3905576 (-) 1359 WP_061326967.1 DNA repair protein RadA Machinery gene
  C7M59_RS18565 (C7M59_18545) - 3905696..3908131 (-) 2436 WP_061326969.1 ATP-dependent Clp protease ATP-binding subunit -
  C7M59_RS18570 (C7M59_18550) - 3908133..3909176 (-) 1044 WP_030037320.1 protein arginine kinase -
  C7M59_RS18575 (C7M59_18555) - 3909181..3909708 (-) 528 WP_003482872.1 UvrB/UvrC motif-containing protein -
  C7M59_RS18580 (C7M59_18560) - 3909731..3910195 (-) 465 WP_080443641.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49755.73 Da        Isoelectric Point: 8.1297

>NTDB_id=280578 C7M59_RS18560 WP_061326967.1 3904218..3905576(-) (radA) [Clostridium botulinum strain Mfbjulcb3]
MAKNKIVYTCQQCGYESIKWVGKCPGCNTWNSMVEEEKQSTNKTIKNINIKSEPKMISTIKSSEYERLDTGIKELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATNYGKVLYVSGEESEEQIKMRADRLKVISKEIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKNNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALISSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTSIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=280578 C7M59_RS18560 WP_061326967.1 3904218..3905576(-) (radA) [Clostridium botulinum strain Mfbjulcb3]
ATGGCTAAAAATAAAATTGTATATACATGTCAACAATGTGGGTACGAATCTATAAAATGGGTAGGAAAATGCCCTGGATG
CAATACTTGGAATAGTATGGTAGAAGAAGAAAAACAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAAC
CTAAAATGATATCCACAATAAAATCCAGTGAATACGAAAGATTAGATACTGGAATAAAGGAACTAAATAGAGTTTTAGGT
GGTGGTATAGTTAAGGGATCTCTAACTCTCATATCTGGCTCTCCTGGAATAGGAAAGTCCACAATACTTTTACAAGCTGC
TAATAACATTGCTACTAATTATGGTAAGGTTTTATATGTATCAGGAGAAGAATCAGAAGAGCAAATAAAAATGAGGGCAG
ATAGACTTAAGGTAATATCAAAAGAAATATACATACTTTCAGAAACAAATGTAGATATAATAAAAGAACACATTACGACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACTCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAGTGTTCTAACGATATTATGCTTATAAGCAAAAATAATAACATACCTTTCTTTATAGTGGCACATGTTA
CAAAGCAAGGAGAATTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAAGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATAGGAGTTTTTGAAATGGCAGGAGA
AGGACTATTAGAAATAAGTAACCCTTCCGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGTACTAGACCAATACTTATAGAAATACAAGCGTTGGTAAGTGAAACAAAAGCTTATATGCCAAGAAGA
ACAGCAGTAGGGGTAGACACAGCTAGATTGAATTTAATATTAGCCGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGCGATGTATATGTTAATGTAGTAGGAGGGTTAGATTTAACAGGGACTTTTGCTGATTTAGGATTAGCATTAGCTTTAA
TATCTAGTGCTAAATCAAAAGATATAAAACTACCTAAAGTTTTAGTAGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGCATAAAAATGGGATTTACCAGTATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

AAA domain (AAA_25)

(60-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.502
  radA Streptococcus pneumoniae Rx1

48.899

100

0.491
  radA Streptococcus pneumoniae D39

48.899

100

0.491
  radA Streptococcus pneumoniae R6

48.899

100

0.491
  radA Streptococcus pneumoniae TIGR4

48.899

100

0.491
  radA Streptococcus mitis NCTC 12261

48.899

100

0.491
  radA Streptococcus mitis SK321

48.899

100

0.491

Multiple sequence alignment