Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C7M58_RS18015 Genome accession   NZ_CP027779
Coordinates   3838803..3840161 (+) Length   452 a.a.
NCBI ID   WP_106901344.1    Uniprot ID   -
Organism   Clostridium botulinum strain RF5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3833803..3845161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M58_RS17995 (C7M58_18045) - 3834184..3834648 (+) 465 WP_003482870.1 CtsR family transcriptional regulator -
  C7M58_RS18000 (C7M58_18050) - 3834671..3835198 (+) 528 WP_003482872.1 UvrB/UvrC motif-containing protein -
  C7M58_RS18005 (C7M58_18055) - 3835203..3836246 (+) 1044 WP_030037320.1 protein arginine kinase -
  C7M58_RS18010 (C7M58_18060) - 3836248..3838683 (+) 2436 WP_106901345.1 ATP-dependent Clp protease ATP-binding subunit -
  C7M58_RS18015 (C7M58_18065) radA 3838803..3840161 (+) 1359 WP_106901344.1 DNA repair protein RadA Machinery gene
  C7M58_RS18020 (C7M58_18070) disA 3840180..3841241 (+) 1062 WP_030037313.1 DNA integrity scanning diadenylate cyclase DisA -
  C7M58_RS18025 (C7M58_18075) - 3841273..3841671 (-) 399 WP_003491260.1 hypothetical protein -
  C7M58_RS18030 (C7M58_18080) - 3842072..3843172 (+) 1101 WP_003482883.1 PIN/TRAM domain-containing protein -
  C7M58_RS18035 (C7M58_18085) ispD 3843174..3843863 (+) 690 WP_106901343.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49769.80 Da        Isoelectric Point: 8.3460

>NTDB_id=280560 C7M58_RS18015 WP_106901344.1 3838803..3840161(+) (radA) [Clostridium botulinum strain RF5]
MAKNKIVYTCQQCGYESIKWVGKCPGCNTWNSMVEEEKQSTNKTIKNINIKSEPKMISTIKSSEYERLDTGIKELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKVLYVSGEESEEQIKMRADRLKVISKEIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKNNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALISSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTSIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=280560 C7M58_RS18015 WP_106901344.1 3838803..3840161(+) (radA) [Clostridium botulinum strain RF5]
ATGGCTAAAAATAAAATTGTATATACATGTCAGCAATGTGGGTACGAATCTATAAAATGGGTAGGAAAATGCCCTGGATG
TAATACTTGGAATAGTATGGTAGAAGAAGAAAAACAATCAACTAATAAGACTATAAAGAATATAAATATTAAATCAGAAC
CTAAAATGATATCCACAATAAAATCCAGTGAATACGAAAGATTAGATACTGGAATAAAGGAACTAAATAGAGTTTTAGGT
GGTGGTATAGTTAAAGGATCTTTAACTCTTATATCTGGCTCTCCTGGAATAGGAAAGTCCACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGGTTTTATATGTATCAGGAGAAGAATCAGAAGAGCAAATAAAAATGAGGGCAG
ATAGACTTAAGGTAATATCAAAAGAAATATACATACTTTCAGAAACAAATGTAGATATAATAAAAGAACACATTACGACA
ATTAATCCTAAATTTGTTATAATAGATTCCATACAGACTCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAGTGTTCTAACGATATTATGCTTATAAGCAAAAATAATAACATACCTTTCTTTATAGTGGCACATGTTA
CAAAGCAAGGAGAATTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAAGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATAGGAGTTTTTGAAATGGCAGGAGA
AGGACTATTAGAAATAAGTAACCCTTCCGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGTACTAGACCAATACTTATAGAAATACAAGCGTTGGTAAGTGAAACAAAAGCTTATATGCCAAGAAGA
ACAGCAGTAGGAGTAGACACAGCTAGATTAAATTTAATATTAGCCGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGCGATGTATATGTTAATGTAGTAGGAGGGTTAGATTTAACAGGGACTTTTGCTGATTTAGGATTAGCATTAGCTTTAA
TATCTAGTGCTAAATCAAAAGATATAAAATTACCTAAAGTTTTAGTAGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGCATAAAAATGGGATTTACCAGTATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTCTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-217)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.502
  radA Streptococcus pneumoniae Rx1

48.899

100

0.491
  radA Streptococcus pneumoniae D39

48.899

100

0.491
  radA Streptococcus pneumoniae R6

48.899

100

0.491
  radA Streptococcus pneumoniae TIGR4

48.899

100

0.491
  radA Streptococcus mitis NCTC 12261

48.899

100

0.491
  radA Streptococcus mitis SK321

48.899

100

0.491

Multiple sequence alignment