Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C7M56_RS18160 Genome accession   NZ_CP027777
Coordinates   3856378..3857736 (+) Length   452 a.a.
NCBI ID   WP_159036067.1    Uniprot ID   -
Organism   Clostridium botulinum strain Mfbjulcb8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3851378..3862736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M56_RS18140 (C7M56_18250) - 3851759..3852223 (+) 465 WP_003482870.1 CtsR family transcriptional regulator -
  C7M56_RS18145 (C7M56_18255) - 3852246..3852773 (+) 528 WP_003482872.1 UvrB/UvrC motif-containing protein -
  C7M56_RS18150 (C7M56_18260) - 3852778..3853821 (+) 1044 WP_159036066.1 protein arginine kinase -
  C7M56_RS18155 (C7M56_18265) - 3853823..3856258 (+) 2436 WP_042384055.1 ATP-dependent Clp protease ATP-binding subunit -
  C7M56_RS18160 (C7M56_18270) radA 3856378..3857736 (+) 1359 WP_159036067.1 DNA repair protein RadA Machinery gene
  C7M56_RS18165 (C7M56_18275) disA 3857755..3858816 (+) 1062 WP_030037313.1 DNA integrity scanning diadenylate cyclase DisA -
  C7M56_RS18170 (C7M56_18280) - 3858848..3859246 (-) 399 WP_030037311.1 hypothetical protein -
  C7M56_RS18175 (C7M56_18285) - 3859647..3860747 (+) 1101 WP_003482883.1 PIN/TRAM domain-containing protein -
  C7M56_RS18180 (C7M56_18290) ispD 3860749..3861438 (+) 690 WP_003482885.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49751.70 Da        Isoelectric Point: 8.3489

>NTDB_id=280539 C7M56_RS18160 WP_159036067.1 3856378..3857736(+) (radA) [Clostridium botulinum strain Mfbjulcb8]
MAKNKIVYTCQQCGYESIKWVGKCPGCNTWNSMVEEEKQSTNKTIKNINIKSEPKMISTIKSSEYERLDTGIKELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKVLYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMHISKNNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALISSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTSIIIPDRNKDKISAKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=280539 C7M56_RS18160 WP_159036067.1 3856378..3857736(+) (radA) [Clostridium botulinum strain Mfbjulcb8]
ATGGCTAAAAATAAAATTGTATATACATGTCAGCAATGTGGGTACGAATCTATAAAATGGGTAGGAAAATGCCCAGGATG
TAATACTTGGAATAGTATGGTAGAGGAAGAAAAACAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAAC
CTAAAATGATATCCACAATAAAATCCAGTGAATACGAAAGATTAGATACTGGAATAAAGGAACTAAATAGAGTTTTAGGT
GGTGGTATAGTTAAGGGATCTCTAACTCTTATATCTGGCTCTCCTGGAATAGGAAAGTCCACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGGTTTTATATGTATCAGGAGAAGAATCAGAAGAGCAAATAAAAATGAGGGCAG
ATAGACTTAAGGTAATATCAAAAGATATATACATACTTTCAGAAACAAATGTGGATATAATAAAAGAACACATTACGACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACTCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAGTGTTCTAACGATATTATGCATATAAGCAAAAATAATAACATACCTTTCTTTATAGTGGCACATGTTA
CAAAGCAAGGAGAATTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAAGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTCGGAACTACAAGTGAAATAGGAGTTTTTGAAATGGCAGGAGA
AGGACTATTAGAAATAAGTAACCCTTCCGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGCACTAGACCAATACTTATAGAAATACAAGCGTTGGTAAGTGAAACAAAAGCTTATATGCCAAGAAGA
ACAGCAGTAGGAGTAGACACAGCTAGATTAAATTTAATATTAGCGGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGCGATGTATATGTTAATGTAGTAGGAGGGTTAGATTTAACAGGGACTTTTGCTGATTTAGGATTAGCATTAGCTTTAA
TATCTAGTGCTAAATCAAAAGATATAAAATTACCTAAAGTTTTAGTAGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGCATAAAAATGGGATTTACCAGTATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGCTAAAAATAATGTTAATCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.502
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment