Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C7M79_RS18060 Genome accession   NZ_CP027775
Coordinates   3886235..3887593 (+) Length   452 a.a.
NCBI ID   WP_003491262.1    Uniprot ID   A0AAU8Z0H9
Organism   Clostridium botulinum strain MFBjulcb1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3881235..3892593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M79_RS18040 (C7M79_18395) - 3881616..3882080 (+) 465 WP_003491268.1 CtsR family transcriptional regulator -
  C7M79_RS18045 (C7M79_18400) - 3882103..3882630 (+) 528 WP_003491265.1 UvrB/UvrC motif-containing protein -
  C7M79_RS18050 (C7M79_18405) - 3882635..3883678 (+) 1044 WP_003491264.1 protein arginine kinase -
  C7M79_RS18055 (C7M79_18410) - 3883680..3886115 (+) 2436 WP_003491263.1 ATP-dependent Clp protease ATP-binding subunit -
  C7M79_RS18060 (C7M79_18415) radA 3886235..3887593 (+) 1359 WP_003491262.1 DNA repair protein RadA Machinery gene
  C7M79_RS18065 (C7M79_18420) disA 3887612..3888673 (+) 1062 WP_003491261.1 DNA integrity scanning diadenylate cyclase DisA -
  C7M79_RS18070 (C7M79_18425) - 3888705..3889103 (-) 399 WP_003491260.1 hypothetical protein -
  C7M79_RS18075 (C7M79_18430) - 3889504..3890604 (+) 1101 WP_003491258.1 PIN/TRAM domain-containing protein -
  C7M79_RS18080 (C7M79_18435) ispD 3890606..3891295 (+) 690 WP_003491256.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49683.66 Da        Isoelectric Point: 8.3467

>NTDB_id=280520 C7M79_RS18060 WP_003491262.1 3886235..3887593(+) (radA) [Clostridium botulinum strain MFBjulcb1]
MAKNKIVYTCQQCGYESIKWVGKCPGCNTWNSMVEEEKQSTNKTIKNINIKSEPKMISTIKSSEYERLDTGIKELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATNYGKVLYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKNNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALISSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTSIIIPDRNKGKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=280520 C7M79_RS18060 WP_003491262.1 3886235..3887593(+) (radA) [Clostridium botulinum strain MFBjulcb1]
ATGGCTAAAAATAAAATTGTATATACATGTCAGCAATGTGGGTACGAATCTATAAAATGGGTGGGAAAATGCCCTGGATG
TAATACTTGGAATAGTATGGTAGAAGAAGAAAAACAATCAACTAATAAGACTATAAAGAACATAAATATTAAATCAGAAC
CTAAAATGATATCCACAATAAAATCCAGTGAATACGAAAGATTAGATACTGGAATAAAGGAACTAAATAGAGTTTTAGGT
GGTGGTATAGTTAAGGGATCTCTAACTCTTATATCTGGCTCTCCTGGGATAGGAAAGTCCACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAATTATGGTAAGGTTTTATATGTATCAGGGGAAGAATCAGAAGAGCAAATAAAAATGAGGGCAG
ATAGACTCAAGGTAATATCAAAAGATATATACATACTTTCAGAAACAAATGTGGATATAATAAAAGAACACATTACGACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACTCTTTTTAAAAGTGAACTATCCTCAGCCCCAGGTACTGTGTC
TCAAGTTAGACAGTGTTCTAACGATATTATGCTTATAAGCAAAAATAATAACATACCTTTCTTTATAGTGGCACATGTTA
CAAAGCAAGGAGAATTAGCGGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAAGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTCGGAACTACAAGTGAAATAGGAGTTTTTGAAATGGCAGGAGA
AGGACTATTAGAAATAAGTAACCCTTCCGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGTACTAGACCAATACTTATAGAAATACAAGCGTTGGTAAGTGAAACAAAAGCTTATATGCCAAGAAGA
ACAGCAGTAGGAGTAGACACAGCTAGATTGAATTTAATATTAGCCGTGTTAGAAAAAAAATTAAATATACCTTTTTATAA
TTGCGATGTATATGTTAATGTAGTAGGAGGGTTAGATTTAACAGGGACTTTTGCTGATTTAGGATTAGCATTAGCTTTAA
TATCTAGTGCTAAATCAAAAGATATAAAATTACCTAAAGTTTTAGTAGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGCATAAAAATGGGATTTACCAGTATAATTATACCTGATAGAAATAA
AGGCAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

AAA domain (AAA_25)

(60-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.89

100

0.5
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment