Detailed information NTG0280213

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C4J99_RS04050
• Genome accession: NZ_CP027758
• Coordinates: 910148..911014 (-)
• Length: 288 a.a.
• NCBI ID: WP_124399319.1
• Uniprot ID: -
• Organism: Pseudomonas synxantha strain R2-54-08W
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 905148..916014

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4J99_RS04015 (C4J99_0811)	-	905952..907250 (-)	1299	WP_124399315.1	NAD(P)/FAD-dependent oxidoreductase	-
C4J99_RS04020 (C4J99_0812)	-	907311..907484 (-)	174	WP_124399316.1	DUF3094 family protein	-
C4J99_RS04025 (C4J99_0813)	-	907573..908202 (-)	630	WP_043049309.1	DUF1780 domain-containing protein	-
C4J99_RS04030 (C4J99_0814)	-	908365..909054 (+)	690	WP_124399317.1	energy-coupling factor ABC transporter permease	-
C4J99_RS04035 (C4J99_0815)	-	909119..909334 (+)	216	WP_003171680.1	hypothetical protein	-
C4J99_RS04040 (C4J99_0816)	yacG	909331..909531 (-)	201	WP_003188437.1	DNA gyrase inhibitor YacG	-
C4J99_RS04045 (C4J99_0817)	coaE	909528..910151 (-)	624	WP_124399318.1	dephospho-CoA kinase	-
C4J99_RS04050 (C4J99_0818)	pilD	910148..911014 (-)	867	WP_124399319.1	prepilin peptidase	Machinery gene
C4J99_RS04055 (C4J99_0819)	pilC	911011..912219 (-)	1209	WP_124399320.1	type II secretion system F family protein	Machinery gene
C4J99_RS04060 (C4J99_0820)	-	912421..912834 (+)	414	WP_124399321.1	pilin	-
C4J99_RS04070 (C4J99_0822)	-	913122..913427 (+)	306	WP_124399322.1	DUF6388 family protein	-
C4J99_RS04075 (C4J99_0823)	nadC	913547..914395 (-)	849	WP_124399323.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31788.18 Da
• Isoelectric Point: 8.1654

>NTDB_id=280213 C4J99_RS04050 WP_124399319.1 910148..911014(-) (pilD) [Pseudomonas synxantha strain R2-54-08W]
MILLLTERPWFFVAMALVLGLIAGSFLNVLVWRLPKMLEREWRAQAHEILGLPAASAGPAYNLMRPNSCCTRCEQPIRPW
ENIPLLSYLLLRGRCAHCREPISLRYPLTELACALITATVAWHFGFGWQAGAVLLLSWGLLGLSLIDLDHQLLPDALVLP
LLWLGLILNSADLLATLPDALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMASLLGLI
AGLILLRLRKAPVSAPMPFGPCLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=280213 C4J99_RS04050 WP_124399319.1 910148..911014(-) (pilD) [Pseudomonas synxantha strain R2-54-08W]
TTGATCCTGCTGCTGACCGAACGGCCCTGGTTTTTTGTTGCCATGGCACTGGTGCTGGGCCTGATCGCCGGCAGTTTCCT
CAATGTACTGGTGTGGCGGCTGCCAAAAATGCTCGAGCGCGAATGGCGTGCCCAGGCCCATGAAATCCTCGGCTTGCCTG
CCGCATCCGCCGGGCCGGCCTATAATCTGATGCGCCCGAATTCCTGCTGCACCCGTTGCGAACAGCCGATTCGGCCCTGG
GAAAATATTCCACTGCTCAGCTACCTGTTGCTCAGGGGGCGTTGTGCCCATTGCCGTGAGCCCATCAGCCTTCGTTATCC
CTTAACCGAGCTGGCCTGCGCGTTGATCACGGCCACTGTTGCCTGGCACTTCGGTTTTGGCTGGCAGGCCGGCGCGGTGC
TGTTATTGAGCTGGGGCTTACTGGGCCTGAGCCTGATTGACCTCGACCATCAATTGTTACCGGATGCGCTGGTGCTGCCG
CTGCTATGGCTGGGACTCATCCTGAACAGCGCCGACCTGCTGGCGACCTTGCCCGATGCGCTGTGGGGCGCGGTCATTGG
CTACATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTCACCGGTAAAGATGGCATGGGCCATGGCGACTTCAAGT
TATTGGCATTACTGGGGGCCTGGGGTGGGTGGCAGATACTGCCGATGACATTGCTGATGGCCTCACTGTTGGGCTTGATT
GCCGGGCTGATTCTATTGCGTTTGCGCAAGGCCCCGGTGTCAGCACCAATGCCATTTGGTCCCTGCCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.5	97.222	0.51
pilD	Vibrio campbellii strain DS40M4	50.178	97.569	0.49
pilD	Acinetobacter baumannii D1279779	48.162	94.444	0.455
pilD	Acinetobacter nosocomialis M2	48.162	94.444	0.455
pilD	Neisseria gonorrhoeae MS11	49.615	90.278	0.448