Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9C08_RS05455 Genome accession   NZ_CP032872
Coordinates   1038891..1039340 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain 2KL1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1033891..1044340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C08_RS05415 (D9C08_05415) - 1034231..1034428 (+) 198 WP_121509395.1 hypothetical protein -
  D9C08_RS05420 (D9C08_05420) yckC 1034448..1034903 (+) 456 WP_014478793.1 RDD family protein -
  D9C08_RS05425 (D9C08_05425) yckD 1034985..1035317 (+) 333 WP_014478794.1 YckD family protein -
  D9C08_RS05430 (D9C08_05430) - 1035445..1035867 (+) 423 WP_014478795.1 MarR family transcriptional regulator -
  D9C08_RS05435 (D9C08_05435) - 1035855..1036175 (+) 321 WP_014478796.1 DUF3147 family protein -
  D9C08_RS05440 (D9C08_05440) - 1036194..1036923 (+) 730 Protein_1013 AAA family ATPase -
  D9C08_RS05445 (D9C08_05445) bglC 1036996..1038429 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  D9C08_RS05450 (D9C08_05450) nin/comJ 1038466..1038864 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  D9C08_RS05455 (D9C08_05455) nucA/comI 1038891..1039340 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  D9C08_RS05460 (D9C08_05460) tlpC 1039508..1041229 (-) 1722 Protein_1017 methyl-accepting chemotaxis protein TlpC -
  D9C08_RS05465 (D9C08_05465) hxlB 1041340..1041897 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  D9C08_RS05470 (D9C08_05470) hxlA 1041903..1042535 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  D9C08_RS05475 (D9C08_05475) hxlR 1042764..1043126 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=280192 D9C08_RS05455 WP_014475770.1 1038891..1039340(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain 2KL1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=280192 D9C08_RS05455 WP_014475770.1 1038891..1039340(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain 2KL1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987